Glycine, USP Grade

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Description

GoldBio’s Glycine (USP Grade) is a versatile, high-purity amino acid used across molecular biology, biochemistry, and cell culture research. As both a component of electrophoresis buffer systems (such as Tris-Glycine buffers for protein gel electrophoresis) and a metabolic nutrient in defined cell culture media, glycine supports critical research applications. 

Glycine is primarily valued for its role in polyacrylamide gel buffer systems and as a dietary amino acid for microbial and mammalian cell growth.


Its zwitterionic nature and high-water solubility make it ideal for use in buffer systems for electrophoresis and metabolic studies. With proven reliability in laboratory protocols, GoldBio's glycine offers the consistency and flexibility researchers need for everything from Western blotting (as a buffer constituent) to metabolomics.

 

 

Common Applications Using Glycine

(Click each for more information)

Component of Biological Buffers (e.g., Glycine-HCl, Tris-Glycine)
  • Purpose: To maintain pH stability in biochemical and electrophoresis applications.
  • How It Works: Glycine acts as a zwitterionic buffering agent, especially effective in acidic environments (e.g., Glycine-HCl buffer, around pH 2 to 3.5) and participates in ion equilibrium systems in alkaline buffers such as Tris-Glycine (used in SDS-PAGE). Its amino and carboxyl groups help stabilize pH changes near its pKa values. Glycine’s buffering action depends on the equilibrium between its protonated and deprotonated forms.
  • Applications: SDS-PAGE running buffers, Western blotting transfer buffers, and protein purification protocols.

 

Nutrient Supplement in Microbial and Mammalian Cell Culture
  • Purpose: To provide a source of carbon and nitrogen to support microbial growth or optimize mammalian cell health.
  • How It Works: Glycine is metabolized by many organisms and supports biosynthesis of purines, glutathione, and other critical biomolecules.
  • Applications: Used in defined media for bacterial fermentation, CHO cell culture, and metabolic studies.

Eagle, H. (1955). Nutrition needs of mammalian cells in tissue culture. Science, 122(3168), 501-504.

Precursor in One-Carbon and Glutathione Metabolism Studies
  • Purpose: To investigate cellular metabolism, oxidative stress responses, and folate pathway function.
  • How It Works: Glycine donates one-carbon units and is required for biosynthesis of nucleotides and glutathione.
  • Applications: Used in metabolic flux analysis, isotope tracing, and oxidative stress research.

Wang, W. et al. (2019). Cell Metabolism, 29(4), 835–849. https://doi.org/10.1016/j.cmet.2018.12.020

 

Key Benefits:

·         Dual functionality as amino acid and buffer: Useful in a wide range of biochemical assays.

·         High solubility and purity: Ensures reliable results with minimal background interference.

·         Cost-effective and stable: An essential lab staple in daily protocols.

 

Storage/Handling:

Store powder at room temperature.

 

Glycine, USP Grade

View Sizes & Pricing

Catalog Number:
G-630-100
CAS Number:
56-40-6
$27.00

For research use only. Not for food, drug, household, or cosmetic use.
Availability:
In stock
Shipping:
$14.99 Ground shipping (In continental US only.)

    Description

    GoldBio’s Glycine (USP Grade) is a versatile, high-purity amino acid used across molecular biology, biochemistry, and cell culture research. As both a component of electrophoresis buffer systems (such as Tris-Glycine buffers for protein gel electrophoresis) and a metabolic nutrient in defined cell culture media, glycine supports critical research applications. 

    Glycine is primarily valued for its role in polyacrylamide gel buffer systems and as a dietary amino acid for microbial and mammalian cell growth.


    Its zwitterionic nature and high-water solubility make it ideal for use in buffer systems for electrophoresis and metabolic studies. With proven reliability in laboratory protocols, GoldBio's glycine offers the consistency and flexibility researchers need for everything from Western blotting (as a buffer constituent) to metabolomics.

     

     

    Common Applications Using Glycine

    (Click each for more information)

    Component of Biological Buffers (e.g., Glycine-HCl, Tris-Glycine)
    • Purpose: To maintain pH stability in biochemical and electrophoresis applications.
    • How It Works: Glycine acts as a zwitterionic buffering agent, especially effective in acidic environments (e.g., Glycine-HCl buffer, around pH 2 to 3.5) and participates in ion equilibrium systems in alkaline buffers such as Tris-Glycine (used in SDS-PAGE). Its amino and carboxyl groups help stabilize pH changes near its pKa values. Glycine’s buffering action depends on the equilibrium between its protonated and deprotonated forms.
    • Applications: SDS-PAGE running buffers, Western blotting transfer buffers, and protein purification protocols.

     

    Nutrient Supplement in Microbial and Mammalian Cell Culture
    • Purpose: To provide a source of carbon and nitrogen to support microbial growth or optimize mammalian cell health.
    • How It Works: Glycine is metabolized by many organisms and supports biosynthesis of purines, glutathione, and other critical biomolecules.
    • Applications: Used in defined media for bacterial fermentation, CHO cell culture, and metabolic studies.

    Eagle, H. (1955). Nutrition needs of mammalian cells in tissue culture. Science, 122(3168), 501-504.

    Precursor in One-Carbon and Glutathione Metabolism Studies
    • Purpose: To investigate cellular metabolism, oxidative stress responses, and folate pathway function.
    • How It Works: Glycine donates one-carbon units and is required for biosynthesis of nucleotides and glutathione.
    • Applications: Used in metabolic flux analysis, isotope tracing, and oxidative stress research.

    Wang, W. et al. (2019). Cell Metabolism, 29(4), 835–849. https://doi.org/10.1016/j.cmet.2018.12.020

     

    Key Benefits:

    ·         Dual functionality as amino acid and buffer: Useful in a wide range of biochemical assays.

    ·         High solubility and purity: Ensures reliable results with minimal background interference.

    ·         Cost-effective and stable: An essential lab staple in daily protocols.

     

    Storage/Handling:

    Store powder at room temperature.

     

    Product Specifications

    Catalog ID: G-630
    CAS #: 56-40-6
    Formula: C2H5NO2
    MW: 75.07 g/mol
    Storage/handling: Store at room temperature.
    PubChem Chemical ID: 750

    CITATIONS

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