Description
Glyoxal (High Density) 6% Crosslinked Agarose Beads are designed for high-capacity covalent immobilization of proteins and other biomolecules used in affinity purification, enzyme immobilization, and custom chromatography. The increased density of glyoxal functional groups enables efficient coupling of affinity ligands through primary amines, allowing stable attachment without requiring resin activation.
The high-density glyoxal surface is well suited for preparing affinity resins, immobilized enzyme supports, antibody purification media, and custom chromatography matrices where greater ligand loading is beneficial. The increased number of reactive glyoxal groups provides more coupling sites for affinity ligands, making the resin an excellent choice for applications requiring higher immobilization capacity.
The resin contains approximately 40-60 µmol glyoxal groups per mL of gel and provides a convenient alternative to CNBr-activated agarose while offering stable covalent attachment. Glyoxal 6% agarose beads are suitable for FPLC applications at pressures up to 20 kPa. Higher pressures or excessive flow rates may reduce column performance.
Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.
Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.
Choosing High and Low Density Agarose Beads
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High Density Resin
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Low Density Resin
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High Crosslinking
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Low Crosslinking
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Best for smaller proteins
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Best for larger proteins (e.g. antibodies)
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Best for repeated use or FPLC systems
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Best for batch incubations or gravity flow
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Small pores
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Large pores
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Stronger covalent binding of ligands improves ligand stability
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Weaker covalent binding improves ligand mobility after immobilization
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Storage/Handling
Store at 4°C. Do not freeze.
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