Glyoxal (Low Density) 6% Agarose Beads, Crosslinked

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Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (15-25 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.

Choosing High and Low Density Agarose Beads

High Density Resin

Low Density Resin

High Crosslinking

Low Crosslinking

Best for smaller proteins

Best for larger proteins (e.g. antibodies)

Best for repeated use or FPLC systems

Best for batch incubations or gravity flow

Small pores

Large pores

Stronger covalent binding of ligands improves ligand stability

Weaker covalent binding improves ligand mobility after immobilization

Storage/Handling

Store at 4°C. Do not freeze.

Glyoxal (Low Density) 6% Agarose Beads, Crosslinked

View Sizes & Pricing

Catalog Number:
G-302-25
CAS Number:
$152.00

Availability:
2-3 Weeks
Shipping:
Shipping calculated at checkout

    Description

    Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

    Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (15-25 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

    The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

    Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.

    Choosing High and Low Density Agarose Beads

    High Density Resin

    Low Density Resin

    High Crosslinking

    Low Crosslinking

    Best for smaller proteins

    Best for larger proteins (e.g. antibodies)

    Best for repeated use or FPLC systems

    Best for batch incubations or gravity flow

    Small pores

    Large pores

    Stronger covalent binding of ligands improves ligand stability

    Weaker covalent binding improves ligand mobility after immobilization

    Storage/Handling

    Store at 4°C. Do not freeze.

    Product Specifications

    Catalog ID: G-302
    Storage/handling: store at 4°C. Do NOT freeze.

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