Description
Glyoxal (Low Density) 6% Crosslinked Agarose Beads are used to covalently immobilize proteins and other biomolecules for affinity purification, enzyme immobilization, and custom chromatography.
The agarose beads are functionalized with glyoxal groups that react with primary amines, allowing stable attachment of antibodies, enzymes, Protein A, Protein G, and other affinity ligands without requiring resin activation.
The low-density glyoxal surface is useful for immobilizing antibodies, enzymes, Protein A, Protein G, and other affinity ligands when reduced ligand crowding or improved accessibility to binding sites is desired. Lower ligand density can help preserve biomolecular activity for larger proteins or applications where steric hindrance may reduce binding efficiency.
The resin contains approximately 15-25 µmol glyoxal groups per mL of gel and provides a convenient alternative to CNBr-activated agarose while enabling stable covalent attachment of affinity ligands. The lower glyoxal density is well suited for applications where reduced ligand loading or improved accessibility to immobilized biomolecules is desired.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.
Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.
Choosing High and Low Density Agarose Beads
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High Density Resin
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Low Density Resin
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High Crosslinking
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Low Crosslinking
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Best for smaller proteins
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Best for larger proteins (e.g. antibodies)
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Best for repeated use or FPLC systems
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Best for batch incubations or gravity flow
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Small pores
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Large pores
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Stronger covalent binding of ligands improves ligand stability
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Weaker covalent binding improves ligand mobility after immobilization
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Storage/Handling
Store at 4°C. Do not freeze.
