How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
| Catalog ID: | G-211 |
|---|---|
| CAS #: | 50-01-1 |
| MW: | 95.53 g/mol |
| Storage/handling: | Store at room temperature. |
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affinity. This is one of the strongest noncovalent interactions ever...
Eluting biotinylated proteins and DNA from streptavidin beads requires harsh conditions like enzymatic digestion, extreme pH, organic solvents, and heat with excess biotin. Deciding which...
Proteins are extracted from inclusion bodies using solubilizing salts like guanidine and reducing agents like TCEP. The extracted proteins are then purified and refolded. Functional...
Salting in is adding salt to a solution to enhance the solubility of a protein, whereas salting out is adding salt to precipitate a protein....