How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are a really great tool for purifying his-tagged recombinant proteins. When leveraging the interaction between nickel ions and his-tags for affinity purification,...
| Catalog ID: | G-211 |
|---|---|
| CAS #: | 50-01-1 |
| Formula: | CH5N3∙HCl |
| MW: | 95.53 g/mol |
| Storage/handling: | Store at room temperature. |
Nickel agarose beads are a really great tool for purifying his-tagged recombinant proteins. When leveraging the interaction between nickel ions and his-tags for affinity purification,...
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Salt is a major component in protein purification buffers, and optimizing this important parameter can make or break a purification step. But, did you know that...