Description
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.
Product Specifications
HEPPSO;
N-(Hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid;
N-Cyclohexyltaurine;
ULTRA PURE GRADE
Formula: C9H20N2O5S
MW: 268.33 g/mol
PubChem Chemical ID: 100205
Storage/Handling: Store at room temperature.
Video: How to make a 1M HEPPSO Buffer Solution
