Description
The HRE-luciferase plasmid is designed for use in studies related to hypoxia (low oxygen) or oxygen-regulated gene expression. It contains a Hypoxia Response Element (HRE) upstream of a luciferase reporter gene. PTEN regulates p300-dependent hypoxia-inducible factor 1 transcriptional activity through Forkhead transcription factor 3a (FOXO3a).
The HRE-Luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio.
Product Specifications
Concentration: 0.2 μg/μL in TE
Volume: 50 μL
Backbone size: 5820 bp
Vector type: Cloning vector
Promoter: Mini-TK
Bacterial Resistance(s): Ampicillin
Copy number: High Copy
Vector Map
Sequence
GoldBio Vector Map and Sequence for HRE-Luciferase Plasmid
Storage/Handling
HRE-Luciferase Plasmid DNA is at 0.02 μg/μL concentration in TE buffer.
Store all plasmids at -20°C.
