Description
GoldBio’s Hygromycin B (≥98% HPLC pure) enables high-stringency selection in transformation involving plants, fungi, algae, yeast, and mammalian systems.
Hygromycin B is a powerful aminoglycoside antibiotic used extensively in molecular biology for the selection of genetically engineered cells across diverse research organisms. By inhibiting 30S ribosomal activity, it ensures only cells expressing the hph resistance gene survive, streamlining genetic manipulation with minimal background.
With decades of successful use in CRISPR, Agrobacterium-mediated transformation, lentiviral transduction, and algal engineering, Hygromycin B is a proven tool for transgene selection and stable cell line generation.
Choose GoldBio’s Hygromycin B for efficient, reproducible, and broad-spectrum selection.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS.
N.B. International shipments MUST be shipped by air (including Canada and Mexico). Shipments of 25 g, 50 g and 100 g will be shipped as multiples of 25 g units to qualify for the small quantity exception. 500 g pack sizes are a hazardous shipment and will incur a hazmat fee, which we estimate to be $65.
Common Applications:
(Click each for more information)
Selectable Marker in Eukaryotic and Prokaryotic Transformation
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Purpose: To select genetically modified cells containing the hygromycin resistance gene (hph or hygR).
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How It Works: Hygromycin B inhibits protein synthesis by binding to the 30S ribosomal subunit. Only transformed cells expressing the resistance gene survive under antibiotic pressure.
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Applications: Plant, yeast, mammalian, and bacterial transformation workflows for plasmid or genomic integration screening.
Blochlinger, K., & Diggelmann, H. (1984). Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Molecular and Cellular Biology, 4(12), 2929–2931.
Maintenance of Transgenes in Stable Cell Lines
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Purpose: To preserve stable expression of transgenes or selection cassettes following genomic integration.
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How It Works: Hygromycin B is maintained in growth media to eliminate cells lacking the resistance cassette, ensuring long-term stability of engineered populations.
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Applications: Stable mammalian cell lines such as HEK293 and CHO for CRISPR tools, reporters, and recombinant protein expression.
Sanjana, N. E., Shalem, O., & Zhang, F. (2014). Improved vectors and genome-wide libraries for CRISPR screening. Nature Methods, 11(8), 783–784.
Fungal Transformation and Selection
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Purpose: To genetically manipulate fungi for functional genomics, industrial biotechnology, and pathogenicity studies.
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How It Works: Fungal cells expressing the hph resistance gene survive on hygromycin-containing media following transformation.
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Applications: Transformation of Botrytis cinerea, Neurospora, Aspergillus, and other filamentous fungi for knockout and reporter studies.
Hamada, W., Reignault, P., Bompeix, G., & Boccara, M. (1994). Transformation of Botrytis cinerea with the hygromycin B resistance gene, hph. Current Genetics, 26(3), 251–255.
Selection in Algal and Cyanobacterial Systems
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Purpose: To enable genetic engineering in photosynthetic microbes used in biofuel, metabolic, and environmental research.
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How It Works: Transformed algal or cyanobacterial cells expressing hph survive under hygromycin selection, allowing enrichment of engineered strains.
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Applications: Genetic engineering of Chlamydomonas reinhardtii, Synechocystis, and Anabaena species for synthetic biology applications.
Ladygin, V. G., & Boutanaev, A. M. (2002). Transformation of Chlamydomonas reinhardtii CW15 with the Hygromycin Phosphotransferase Gene as a Selectable Marker. Russian Journal of Genetics, 38, 1009–1014.
Generation of Transgenic Plants
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Purpose: To select plant tissues or calli that have successfully integrated T-DNA carrying hph.
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How It Works: Only transformed plant cells expressing hygromycin resistance regenerate and proliferate under hygromycin selection pressure.
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Applications: Functional genomics and trait engineering in rice, Arabidopsis, tobacco, and other crop or model plant systems.
Hiei, Y., Ohta, S., Komari, T., & Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant Journal, 6(2), 271–282.
Benefit:
- Multi-host compatibility: Applicable to plants, fungi, algae, yeast, and mammalian systems using the same hph gene.
- Efficient selection: High stringency ensures fast elimination of non-transformants, reducing background and saving time.
- Versatile in genome engineering: Compatible with CRISPR/Cas9, stable integration, T-DNA systems, and viral delivery tools.
- Reliable in long-term maintenance: Ideal for preserving stable expression of inserted constructs.
- Backed by decades of literature: A well-characterized selection antibiotic with reproducible performance across systems.
Storage/Handling:
Store desiccated at -20°C.
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