Description
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Human Interleukin-15
IL-15
Interleukin 15
IL15
Human interleukin-15 (IL15) is expressed by the IL15 gene located on chromosome 4. It shares approximately 97% and 73% sequence identity with simian and murine IL15, respectively. Both human IL15 and simian IL15 are active on murine cells. IL15 is secreted by mononuclear phagocytes, especially macrophages following infection by virus. It possesses a variety of biological functions that include the stimulation and maintenance of cellular immune responses, specifically the responses which regulate natural killer (NK) cell and T-cell activation and proliferation. In addition, IL15 shares many biological properties with IL2, including T, B and NK cell-stimulatory activities. IL15 signals through a complex composed of IL2/IL15 receptor beta chain. Although IL15 lacks sequence homology with IL2, it has been shown that both the beta and gamma chains of the IL2 receptor are utilized for IL15 binding and signaling. In addition, an IL15 specific binding protein has been cloned from a mouse T-cell clone.
Formulation: Sterile filtered white lyophilized powder.
Sequence: NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS
MW: ~12.9 kDa (114 amino acids)
Purity: >97% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0 × 106 IU/mg.
Accession Number: P40933
HGNC ID: 5977
Source: Escherichia coli
Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum.
Additional resources for this product may be found at:
OMIM
NOT FOR HUMAN USE!
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