Description GoldBio's IPTG EZ Pak™ for 1 M Solution provides a fast, convenient way to prepare a set volume of sterile 1 M IPTG without weighing IPTG, calculating the required amount, or assembling separate filtration supplies.
Each kit contains pre-weighed GoldBio IPTG, a sterile filter, and a sterile container for the finished solution. Simply add the specified amount of deionized water, filter the solution, and transfer it to the provided labeled bottle. The resulting sterile 1 M IPTG solution is ready for use.
The EZ Pak™ is available for preparing 10 mL, 50 mL, or 100 mL of 1 M IPTG solution. The 10 mL size includes a syringe filter for sterilization, while the 50 mL and 100 mL sizes are designed for vacuum filtration and require a vacuum manifold. This premeasured format helps simplify routine stock solution preparation and provides a consistent starting concentration between preparations.
IPTG is a non-metabolizable galactose analog commonly used to induce expression from lac-controlled promoters in E. coli . It binds the lac repressor and allows transcription of genes controlled by the lac operon. This makes IPTG widely used for recombinant protein expression and for blue/white colony screening when paired with chromogenic substrates such as X-Gal.
The IPTG supplied in GoldBio's EZ Pak™ is synthetic and non-animal origin.
IPTG is commonly used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies.
Kit Components
Pre-weighed GoldBio IPTG for preparation of a 1 M solution
Applications
Common Applications for IPTG
(Click each for more information)
Induction of Protein Expression in Lac Operon-Based Systems
Purpose : To induce recombinant protein expression in bacterial systems, particularly Escherichia coli .
How It Works : IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.
Applications : High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.
Studier, F. W., & Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology , 189(1), 113–130.
Blue/White Screening in Cloning
Purpose : To differentiate recombinant clones from non-recombinant colonies during cloning workflows.
How It Works : IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact lacZα appear blue, while recombinant colonies with disrupted lacZ remain white.
Applications : Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.
Vieira, J., & Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene , 19(3), 259–268.
Study of Gene Regulation and Promoter Function
Purpose : To investigate transcriptional regulation and promoter-controlled gene expression.
How It Works : IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.
Applications : Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.
Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... & Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli . Nature Methods , 3(8), 623–628.
Metabolic Engineering and Pathway Control
Purpose : To enable temporal regulation of enzyme expression in engineered metabolic pathways.
How It Works : Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.
Applications : Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.
Zhang, F., Carothers, J. M., & Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature Biotechnology , 30(4), 354–359.
Conditional Gene Expression in Synthetic Biology
Purpose : To provide external control over synthetic gene circuits and regulatory networks.
How It Works : LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.
Applications : Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.
Elowitz, M. B., & Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature , 403(6767), 335–338.
Key Benefits
No Calculations or Weighing: Pre-weighed IPTG eliminates the need to calculate and measure the amount required to prepare a 1 M stock solution.
Convenient Sterile Preparation: The included filtration device and sterile container provide the materials needed to prepare and store a sterile IPTG solution.
Consistent 1 M Stock Preparation: Each EZ Pak™ is premeasured for a defined final volume, helping researchers prepare the same stock concentration each time.
Multiple Preparation Volumes: Available for preparing 10 mL, 50 mL, or 100 mL of 1 M IPTG to accommodate different laboratory usage levels.
Ready for Common IPTG Applications: Once reconstituted and filtered, the solution can be used for recombinant protein induction, bacterial media, blue/white screening, and other IPTG-based research applications.
Synthetic, Non-Animal Origin IPTG: Uses GoldBio IPTG produced from synthetic, non-animal sources.
Storage/Handling
Store unreconstituted IPTG desiccated at -20°C.
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