IPTG EZ Pak™ for 1 M Solution

Request Lot Specific COA

Citation Search Tool

Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

The 50 ml and 100 ml require a vacuum manifold for easy vacuum filtering, while the 10 ml pack size comes with a syringe filter.

IPTG is commonly used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies.

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).

GoldBio IPTG is made from a synthetic, non-animal origin.

PubChem Chemical ID: 656894

IPTG EZ Pak™ for 1 M Solution

View Sizes & Pricing

Catalog Number:
I2481-EZ10
CAS Number:
367-93-1
$80.00

Availability:
In stock
Shipping:
Shipping calculated at checkout

    Description

    The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

    The 50 ml and 100 ml require a vacuum manifold for easy vacuum filtering, while the 10 ml pack size comes with a syringe filter.

    IPTG is commonly used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies.

    IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

    IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).

    GoldBio IPTG is made from a synthetic, non-animal origin.

    PubChem Chemical ID: 656894

    Product Specifications

    Catalog ID: I2481-EZ
    CAS #: 367-93-1
    MW: 238.30 g/mol
    Storage/handling: Store desiccated at -20°C.

    CITATIONS

    Featured Articles

    • How to make IPTG Stock Solution - Banner Image

      How to Make an IPTG Stock Solution

      Making IPTG stock solution involves weighing out IPTG powder, pouring it into a conical tube or cylinder, and adding deionized water to the desired volume....

      Read more

    • IPTG vs. Auto-Induction thumbnail image

      IPTG Induction vs Auto-Induction

      IPTG and auto-induction are two ways to induce protein expression in bacteria. They work similarly, but have different trade-offs in terms of convenience. While IPTG...

      Read more

    • IPTG optimization thumbnail - iptg tubes

      Optimizing Protein Expression with IPTG Induction

      The final concentration of IPTG used for induction varies from 0.1 to 1.0 mM, with 0.5 or 1.0 mM most frequently used. For proteins with...

      Read more

    • What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*

      What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*

      Expression systems are important tools in molecular biology, enabling researchers to produce proteins for research, diagnostics, and therapeutics. Examples of E. coli protein expression strains...

      Read more

    • How Does IPTG Induction Work?

      How Does IPTG Induction Work?

      Though you’ve used IPTG for your experiments, you may be unfamiliar with the mechanism of IPTG induction. This article provides a quick review on the...

      Read more

    Login

    Forgot your password?

    Don't have an account yet?
    Create account