Description
GoldBio’s L-Arginine is a high-purity, water-soluble amino acid essential for supporting metabolic, immunological, and signaling functions in biological research. Widely used in cell culture media, nitric oxide production studies, and protein biosynthesis, L-arginine plays critical roles in the urea cycle, T cell metabolism, and cellular growth pathways. With ≥99% purity and excellent solubility, this reagent is ideal for applications in cancer metabolism, immunology, and in vitro diagnostics.
Whether you’re optimizing cell growth, studying NO signaling, or analyzing amino acid flux, GoldBio’s L-Arginine offers dependable performance and consistent quality for your research needs.
Functional Highlights and Mechanism
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L-Arginine serves as a substrate for nitric oxide synthase (NOS) enzymes, leading to production of nitric oxide (NO) and L-citrulline—this pathway is central in vascular biology, signaling, and cellular redox balance.
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Through its NO-generating capacity, L-Arginine can stimulate insulin release in certain contexts via NO-dependent mechanisms.
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As a basic amino acid with a guanidinium side chain, L-Arginine contributes to protein structure, charge interactions, and can influence buffer or formulation systems when used as an additive (for example, in protein stabilization or solubility enhancement) (though not explicitly from the product page, this is well established).
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Because L-Arginine contains a free amino group and guanidino group, it can participate in hydrogen bonding and ionic interactions, which may influence its behaviour in solutions, buffers, and in the presence of other biomolecules.
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Its high solubility and capacity to serve as a component in metabolic or cell culture experiments make it a versatile reagent; using GoldBio’s well-characterized material helps ensure reproducibility and lot-to-lot consistency.
Recommended Applications and Usage Notes
Recommended Applications
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Use as a supplement in cell culture or media formulations where arginine-dependent metabolic pathways are under investigation.
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Incorporation into enzyme assays or metabolic flux studies where nitric oxide generation from L-Arginine is of interest.
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Use in protein formulation or purification buffers when optimizing solubility, aggregation suppression, or ionic stabilization (with appropriate validation).
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Use in general biochemical reagents preparation, e.g., as a defined amino acid source for minimal media or biochemical reaction mixtures.
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Use in research models of vascular biology, signaling, immunology, where arginine/NO pathways are relevant.
Usage Tips and Considerations
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When preparing stock solutions of L-Arginine, ensure correct pH adjustment (arginine is basic), and account for ionic strength contributions to your system.
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Confirm that the reagent is compatible with your downstream assay. Because L-Arginine is positively charged at physiological pH, it may interact with anionic species or affect ionic interactions in your formulation; perform controls if necessary.
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If using in cell culture, ensure that supplementation levels do not inadvertently alter osmolarity or ionic balance of the medium.
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Always reference the lot-specific Certificate of Analysis supplied by GoldBio to verify properties such as assay value, impurities, and storage condition.
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Since the product is labelled for “Research Use Only,” ensure that it is not used for human, veterinary, or clinical applications. Check regulatory/ institutional compliance for your work.
Common Research Applications
(Click each for more information)
Precursor for Nitric Oxide (NO) Biosynthesis
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Purpose: To study nitric oxide signaling in vascular biology, neuroscience, and immunology.
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How It Works: L-Arginine is converted by nitric oxide synthase (NOS) into nitric oxide and citrulline. NO acts as a signaling molecule influencing vasodilation, neurotransmission, and immune response.
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Applications: Endothelial function assays, macrophage activation studies, NO-mediated signaling research.
Moncada, S., & Higgs, A. (1993). The L-arginine–nitric oxide pathway. New England Journal of Medicine, 329(27), 2002–2012.
Supplement in Cell Culture Media
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Purpose: To support cellular metabolism and proliferation in vitro.
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How It Works: L-Arginine is a conditionally essential amino acid involved in protein synthesis, urea cycle function, and production of polyamines and creatine.
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Applications: CHO and HEK cell culture, stem cell media, custom media formulations.
Eagle, H. (1955). The specific amino acid requirements of a human carcinoma cell (strain HeLa) in tissue culture. The Journal of Experimental Medicine, 102(1), 37–48.
Study of Arginine Metabolism in Immunology and Cancer
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Purpose: To investigate how arginine metabolism impacts immune function and tumor growth.
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How It Works: Arginase and NOS compete for L-arginine. In immune cells, arginine levels regulate T cell proliferation, macrophage activation, and tumor microenvironment signaling.
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Applications: Tumor immunology, metabolic pathway analysis, T cell activation assays.
Geiger, R., Rieckmann, J. C., Wolf, T., Basso, C., Feng, Y., Fuhrer, T., ... & Lanzavecchia, A. (2016). L-arginine modulates T cell metabolism and enhances survival and anti-tumor activity. Cell, 167(3), 829–842.
Regulation of the Urea Cycle and Ammonia Detoxification
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Purpose: To study nitrogen metabolism and urea cycle disorders.
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How It Works: In the urea cycle, L-arginine serves as a precursor for urea and ornithine production, facilitating ammonia detoxification.
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Applications: Hepatocyte models, metabolic flux studies, inborn error of metabolism research.
Mew, A., Simpson, K. L., Gropman, A. L., Lanpher, B. C., Chapman, K. A., & Summar, M. L. (2017). Urea cycle disorders overview.
Safety Disclaimer
This product is supplied for laboratory research use only. It is not intended for human or animal therapeutic or diagnostic use. Please refer to the Safety Data Sheet (SDS) provided by GoldBio for full hazard, handling, and disposal instructions. Use appropriate personal protective equipment including lab coat, gloves, and eye protection when handling this reagent. Dispose of any waste solutions in accordance with applicable local, state, and federal regulations.
For RESEARCH USE ONLY and cannot be sold for human consumption!
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