Description
Mix-n-Stain™ CF®750 Antibody Labeling Kit is a near-infrared fluorescent dye that is the brightest fluorescent dye available in its spectrum. Antibody conjugates prepared from CF®750 have by far the best signal-to-noise ratio than antibody conjugates prepared from other spectrally similar dyes.
Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF® dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF® dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF® dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBio CF® antibody labeling kits.
Note: We do not recommend using CF®750 labeled primary antibodies for use with the APC-Cy7 channel. The excitation wavelength for the APC-Cy7 channel is suboptimal for CF®750 and direct immunofluorescence staining using primary antibodies labeled with CF®750 generally results in very low signal.
Technical Summary Abs/Em Maxima: 755/777 nm
Excitation laser line: 633 nm, 635 nm, 680 nm or 685 nm
Extinction Coefficient: 250,000
MW: ~3009 g/mol | | Kit Components
Dye
Mix-n-Stain™ 10X reaction buffer
Mix-n-Stain™ antibody storage buffer
Ultrafiltration vial
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Storage/Handling: Store the kit at -20°C.
Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
