Description GoldBio’s MOPS, Sodium Salt is a zwitterionic biological buffer widely used in molecular biology, protein chemistry, and electrophoresis applications requiring stable pH control near neutrality.
As a member of the Good’s buffer family, MOPS demonstrates low metal ion binding, minimal biological interference, and strong buffering capacity within the physiological pH range, making it suitable for sensitive biochemical and cellular studies.
The sodium salt form offers convenient preparation of buffered solutions and is commonly used in formulations where rapid dissolution and accurate pH adjustment are required.
MOPS sodium salt can be mixed with MOPS free acid to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.
Common Applications
(Click each for more information)
RNA Electrophoresis Buffers
Purpose : To maintain stable pH conditions during denaturing RNA gel electrophoresis.
How It Works : MOPS buffering systems stabilize RNA electrophoresis conditions and support consistent RNA migration patterns.
Applications : RNA gel electrophoresis, northern blot preparation, and RNA integrity analysis.
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press.
Cell Culture Media Buffering
Purpose : To stabilize physiological pH in mammalian and microbial culture systems.
How It Works : MOPS resists pH fluctuations caused by metabolism and environmental changes during cell growth.
Applications : Microbial culture systems and physiological buffer preparation.
Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. M. (1966). Hydrogen ion buffers for biological research. Biochemistry , 5(2), 467–477.
Protein Purification and Enzyme Assays
Purpose : To maintain stable pH conditions that preserve enzyme activity and protein structure.
How It Works : MOPS minimizes biochemical interference while stabilizing pH during purification and assay procedures.
Applications : Protein purification, enzyme kinetics, and biochemical assay development.
Ferguson, W. J., Braunschweiger, K. I., Braunschweiger, W. R., et al. (1980). Hydrogen ion buffers for biological research. Analytical Biochemistry , 104(2), 300–310.
Microbiology and Bacterial Growth Studies
Purpose : To prepare buffered growth media for bacterial and microbial experiments.
How It Works : MOPS stabilizes media pH during microbial growth and metabolism.
Applications : Bacterial culture media, microbial physiology studies, and fermentation research.
Neidhardt, F. C., Bloch, P. L., & Smith, D. F. (1974). Culture medium for enterobacteria. Journal of Bacteriology , 119(3), 736–747.
Biochemical and Diagnostic Buffer Preparation
Purpose : To formulate reproducible buffered solutions for analytical and diagnostic applications.
How It Works : MOPS provides stable buffering with low chemical reactivity near neutral pH.
Applications : Diagnostic reagent preparation, analytical chemistry, and molecular biology buffers.
Good, N. E., & Izawa, S. (1972). Hydrogen ion buffers. Methods in Enzymology , 24, 53–68.
Key Benefits
Maintains stable buffering capacity near physiological pH.
Commonly used in RNA electrophoresis and nucleic acid research applications.
Low metal ion binding and minimal interference with biochemical reactions.
Compatible with protein purification, enzyme assays, and cell culture systems.
Sodium salt form supports convenient preparation of buffered solutions.
Storage/Handling
Store in a dry and well-ventilated place.
Video: How to make a 1M MOPS, Sodium Salt Buffer Stock Solution
VIDEO
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