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Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Product Specifications
| Catalog ID: | R-202 |
|---|---|
| Storage/handling: | Store at 4°C. Do NOT freeze. |
Technical Documentation
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Protocol
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Other
C
Certificate of Analysis
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Safety Data Sheet
CITATIONS
Featured Articles
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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Insoluble Protein Purification: How To Get Proteins Out of Inclusion Bodies?
Proteins are extracted from inclusion bodies using solubilizing salts like guanidine and reducing agents like TCEP. The extracted proteins are then purified and refolded. Functional...
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Conjugating Ligands to Agarose Beads for Affinity Purification
Affinity purification is a frequently used technique for purifying proteins. During affinity purification, affinity tags or intrinsic properties of the proteins are used to bind...
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How to Choose Agarose Resins for Protein Purification
Purifying a target protein is an important step before investigating its structure and function. To purify a target protein from a mixture of substances, column...
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How Column Chromatography Works to Separate Proteins
Without a doubt, protein purification has been an important process in protein research, particularly for investigating the function and structure of a protein. To help...
