Powered by Bioz
What Is a His-Tag?
His-tags are popular affinity tags that are used for protein purification, detection, and many different assays. But if you’re new to the protein biochemistry space,...
Product Specifications
| Catalog ID: | H-355 |
|---|---|
| Storage/handling: | Store at 4°C. Do NOT freeze. |
| UN number: | NA-1993 |
| group number: | III |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
CITATIONS
Featured Articles
-
-
Why Did My Nickel Agarose Beads Lose Their Color?
Nickel agarose beads have a beautiful blue color. This blue color isn’t just for aesthetics; it is also a useful tracker of your purification or...
-
Why Did My Nickel Agarose Beads Turn Black or Brown?
If you’ve purified his-tagged proteins with nickel agarose beads, then you know the characteristic brilliant blue color of these beads. When working with those beads,...
-
Why Are Nickel Agarose Beads Blue?
Many experimental procedures in science have no way to monitor in real time how the procedure is going. Think about it, we’re usually pipetting colorless...
-
How to Strip, Clean, and Recharge Nickel Agarose Beads
Have you ever heard the adage “reduce, reuse, recycle?” When it comes to purifying proteins with agarose beads, we certainly hope you aren’t reducing that;...
-
What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
There are a lot of options when choosing nickel agarose beads for purifying your his-tagged proteins. So, the decision on which beads to use can...
-
Understanding Binding Capacity for Nickel Agarose Beads
If you like to cook, then you know that the proportion of each ingredient in a recipe is really important. For example, if you’re marinating...
-
How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are a really great tool for purifying his-tagged recombinant proteins. When leveraging the interaction between nickel ions and his-tags for affinity purification,...
-
Insoluble Protein Purification: How To Get Proteins Out of Inclusion Bodies?
Have you ever had a problem where you had to try many different potential solutions before you finally found one that worked? Early in my...
-
Regular vs Magnetic Agarose Beads: Key Differences and Best Uses
Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
-
Conjugating Ligands to Agarose Beads for Affinity Purification
Affinity purification is a frequently used technique for purifying proteins. During affinity purification, affinity tags or intrinsic properties of the proteins are used to bind...
-
How Column Chromatography Works to Separate Proteins
Without a doubt, protein purification has been an important process in protein research, particularly for investigating the function and structure of a protein. To help...
