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Why Are Nickel Agarose Beads Blue?
Many experimental procedures in science have no way to monitor in real time how the procedure is going. Think about it, we’re usually pipetting colorless...
Product Specifications
| Catalog ID: | P-301 |
|---|---|
| Storage/handling: | Store at RT. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
CITATIONS
Featured Articles
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are a really great tool for purifying his-tagged recombinant proteins. When leveraging the interaction between nickel ions and his-tags for affinity purification,...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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What is a GST Pull-Down Assay? The Complete Overview with Protocols and Analyses Guides
Molecular interactions are foundational for homeostasis at cellular and organism levels, and the dysregulation of these interactions causes many diseases. That’s why when a scientist...
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Regular vs Magnetic Agarose Beads: Key Differences and Best Uses
Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
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The Purpose of Magnetic Agarose Beads
Have you ever seen a cartoon where a huge magnet is used to pull objects into the sky? Usually, the magnet is intended to secretly...
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How to Perform Immunoprecipitation with Protein A, G, or L Agarose Beads
mmunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to isolate a particular protein of interest out of a complex biochemical mixture...
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Deep Dive into Eluting Antibodies from Protein A, Protein G and Protein L Agarose Beads
If you’re using Protein A, Protein G, or Protein L agarose beads to purify antibodies, then a key step in this process is eluting the...
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All About Protein A Agarose Beads: Their Function and Uses
Protein A is a bacterial protein from the cell wall of Staphylococus aureus that binds to the heavy chains of antibodies. This interaction is leveraged...
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What is Ion Exchange Chromatography Used For? 4 Important Applications for Protein Purification
Ion Exchange Chromatography is routinely sandwiched between affinity and size exclusion steps in an overall protein purification protocol. However, ion exchange is not just some...
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What is ion exchange chromatography?
Have you ever heard the saying “opposites attract”? Well, it turns out this phrase is true in the molecular world as well. Positive and negative...
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Antibody Purification with Affinity Chromatography
Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense when we are infected. Scientists frequently use antibodies as...
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GST Tag, MBP Tag, and SUMO Tag: 3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto your protein of interest while cloning it...
