Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
| Catalog ID: | P-301 | 
|---|---|
| Storage/handling: | Store at RT. | 
        
      The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
        
      Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
        
      Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
        
      Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
        
      Have you ever seen a cartoon where a huge magnet is used to pull objects into the sky? Usually, the magnet is intended to secretly...
        
      mmunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to isolate a particular protein of interest out of a complex biochemical mixture...
        
      If you’re using Protein A, Protein G, or Protein L agarose beads to purify antibodies, then a key step in this process is eluting the...
        
      Protein A is a bacterial protein from the cell wall of Staphylococus aureus that binds to the heavy chains of antibodies. This interaction is leveraged...
        
      Ion Exchange Chromatography is routinely sandwiched between affinity and size exclusion steps in an overall protein purification protocol. However, ion exchange is not just some...
        
      Have you ever heard the saying “opposites attract”? Well, it turns out this phrase is true in the molecular world as well. Positive and negative...
        
      Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense when we are infected. Scientists frequently use antibodies as...
        
      Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto your protein of interest while cloning it...