ProBlock™ Gold Protease Inhibitor Cocktail [100X]

Request Lot Specific COA

Citation Search Tool

Description

ProBlock™ Gold Protease Inhibitor Cocktail [100X] is a ready-to-use liquid cocktail for protecting proteins from degradation during cell and tissue extraction, lysis, and protein purification.

The broad-spectrum formulation combines reversible and irreversible inhibitors targeting serine, cysteine, and other proteases. It is suitable for protein isolation from animal and plant tissues, yeast, and bacteria.

At a 1X concentration in extraction buffer at pH 7 to 8, ProBlock™ Gold inhibits more than 90% of protease activity in GoldBio's mouse pancreas extract test system  (e.g. Mouse Pancreas Extract, 0.5 mg/ml protein).

The 100X liquid format allows you to pipette the amount needed directly into an extraction buffer or lysate without dissolving tablets or preparing individual inhibitor stocks.

Its formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF. Together, these inhibitors provide broader protease coverage than a single inhibitor and help preserve intact proteins for purification and downstream analysis.

ProBlock™ Gold is supplied without EDTA in the primary cocktail, with a separate bottle of EDTA included for optional metalloprotease inhibition. This gives you control over when chelation is appropriate. EDTA can interfere with proteins that depend on Ca²⁺, Mg²⁺, or Mn²⁺ and is incompatible with immobilized metal affinity chromatography unless removed before purification.

Leaving EDTA out also makes the primary cocktail suitable for applications such as isoelectric focusing. When metalloprotease inhibition is needed, the supplied EDTA solution can be added separately to the extraction buffer or lysate.

 

Components:

  • ProBlock™ Gold Protease Inhibitor Cocktail [100X]
  • Optional, separate bottle of EDTA for optimized metalloprotease inhibition

 

Cocktail Protease Inhibitors:

  • AEBSF
  • Aprotinin
  • Bestatin
  • E-64
  • Leupeptin
  • Pepstatin A
  • PMSF

 

Mechanism

ProBlock™ Gold Protease Inhibitor Cocktail [100X] protects proteins by combining inhibitors with complementary mechanisms and protease specificities. This broad-spectrum approach helps prevent degradation from multiple classes of proteases that can be released when cells or tissues are disrupted.

 

 

Comparison

Feature

ProBlock™ Gold [100X]

(Catalog Number: GB-108)

ProBlock™ Gold Extra Strength

(Catalog Number: GB-116)

Best For

Routine protein extraction and purification

More demanding protein extraction and purification where increased inhibitor strength is desired

Core Inhibitors

AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF

AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF

Protease Coverage

Broad-spectrum inhibition of serine, cysteine, and other proteases

Broad-spectrum inhibition with an enhanced dose of protease inhibitors

Metalloprotease Inhibition

Separate EDTA solution included

Separate EDTA and 1,10-phenanthroline monohydrate solutions included

EDTA

Optional

Optional

1,10-Phenanthroline

Not included

Included separately for additional metalloprotease inhibition

Metal-Sensitive Applications

EDTA can be omitted when divalent cations must be preserved

EDTA and 1,10-phenanthroline can be omitted when divalent cations must be preserved

 

 

 

Common Applications

(Click each for more information)

Protein Extraction and Cell Lysis
  • Purpose: To protect proteins from degradation when cells or tissues are disrupted for protein extraction.
  • How It Works: ProBlock™ Gold combines inhibitors that target multiple protease classes released during lysis. Adding the 100X cocktail to extraction buffer helps limit unwanted proteolysis before proteins can be isolated or analyzed.
  • Applications: Mammalian cell lysis, bacterial and yeast protein extraction, plant and animal tissue homogenization, and preparation of whole-cell lysates.

Grabski, A. C. (2009). Advances in preparation of biological extracts for protein purification. Methods in Enzymology, 463, 285–303. https://doi.org/10.1016/S0076-6879(09)63018-4

Recombinant Protein Purification
  • Purpose: To reduce degradation of recombinant proteins during isolation and purification.
  • How It Works: The cocktail inhibits endogenous proteases that can remain active after cell disruption and continue degrading the target protein during subsequent purification steps. The separately supplied EDTA can be omitted when using immobilized metal affinity chromatography.
  • Applications: Affinity chromatography, recombinant protein isolation, enzyme purification, and preparation of proteins for downstream characterization.

Ryan, B. J., & Henehan, G. T. (2013). Overview of approaches to preventing and avoiding proteolysis during expression and purification of proteins. Current Protocols in Protein Science, 71, 5.25.1–5.25.7. https://doi.org/10.1002/0471140864.ps0525s71

Proteomics Sample Preparation
  • Purpose: To preserve the protein composition of cell and tissue samples prior to proteomic analysis.
  • How It Works: Broad protease inhibition limits unwanted protein cleavage after sample collection and lysis, helping preserve proteins before subsequent processing and analysis.
  • Applications: Protein extraction for mass spectrometry, quantitative proteomics, protein profiling, and high-throughput proteomic studies, with inhibitor removal or dilution before protease digestion when required.

Burns, A. P., Zhang, Y. Q., Xu, T., Wei, Z., Yao, Q., Fang, Y., ... & Tao, D. (2021). A universal and high-throughput proteomics sample preparation platform. Analytical Chemistry, 93(24), 8423–8431.

Preparation of Samples for 2D Electrophoresis and Isoelectric Focusing
  • Purpose: To minimize proteolytic changes to proteins before electrophoretic separation while allowing researchers to avoid EDTA when necessary.
  • How It Works: Protease inhibitors help preserve proteins during sample preparation before electrophoresis. Because EDTA is supplied separately from ProBlock™ Gold, the primary cocktail can be used without the chelator for applications where EDTA could interfere with separation or analysis.
  • Applications: Two-dimensional gel electrophoresis, isoelectric focusing, protein profiling, and comparative proteomic analysis.

Castellanos-Serra, L., & Paz-Lago, D. (2002). Proteomics and two-dimensional electrophoresis-Inhibition of unwanted proteolysis during sample preparation: Evaluation of its efficiency in challenge experiments. Electrophoresis-An International Journal, 23(11), 1745–1753.

 

Benefits:

  • Greater Range of Inhibition – 95%+
  • Better than tablets – the ready-to-use, refrigerator-stable, 100X liquid format is more effective and easier to use than traditional tablet products; just pipette exactly the amount you need!
  • No proprietary ingredients – formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A, and PMSF
  • Multiple package sizes
  • EDTA-free formulation is perfect for isoelectric focusing
  • Optional bottle of EDTA is included for optimized metalloprotease inhibition

 

Storage/Handling: 

Store at 4°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

ProBlock™ Gold Protease Inhibitor Cocktail [100X]

View Sizes & Pricing

Catalog Number:
GB-108-2
CAS Number:
$132.00

For research use only. Not for food, drug, household, or cosmetic use.
Availability:
In stock
Shipping:
$14.99 Ground shipping (In continental US only.)

    Description

    ProBlock™ Gold Protease Inhibitor Cocktail [100X] is a ready-to-use liquid cocktail for protecting proteins from degradation during cell and tissue extraction, lysis, and protein purification.

    The broad-spectrum formulation combines reversible and irreversible inhibitors targeting serine, cysteine, and other proteases. It is suitable for protein isolation from animal and plant tissues, yeast, and bacteria.

    At a 1X concentration in extraction buffer at pH 7 to 8, ProBlock™ Gold inhibits more than 90% of protease activity in GoldBio's mouse pancreas extract test system  (e.g. Mouse Pancreas Extract, 0.5 mg/ml protein).

    The 100X liquid format allows you to pipette the amount needed directly into an extraction buffer or lysate without dissolving tablets or preparing individual inhibitor stocks.

    Its formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF. Together, these inhibitors provide broader protease coverage than a single inhibitor and help preserve intact proteins for purification and downstream analysis.

    ProBlock™ Gold is supplied without EDTA in the primary cocktail, with a separate bottle of EDTA included for optional metalloprotease inhibition. This gives you control over when chelation is appropriate. EDTA can interfere with proteins that depend on Ca²⁺, Mg²⁺, or Mn²⁺ and is incompatible with immobilized metal affinity chromatography unless removed before purification.

    Leaving EDTA out also makes the primary cocktail suitable for applications such as isoelectric focusing. When metalloprotease inhibition is needed, the supplied EDTA solution can be added separately to the extraction buffer or lysate.

     

    Components:

    • ProBlock™ Gold Protease Inhibitor Cocktail [100X]
    • Optional, separate bottle of EDTA for optimized metalloprotease inhibition

     

    Cocktail Protease Inhibitors:

    • AEBSF
    • Aprotinin
    • Bestatin
    • E-64
    • Leupeptin
    • Pepstatin A
    • PMSF

     

    Mechanism

    ProBlock™ Gold Protease Inhibitor Cocktail [100X] protects proteins by combining inhibitors with complementary mechanisms and protease specificities. This broad-spectrum approach helps prevent degradation from multiple classes of proteases that can be released when cells or tissues are disrupted.

     

     

    Comparison

    Feature

    ProBlock™ Gold [100X]

    (Catalog Number: GB-108)

    ProBlock™ Gold Extra Strength

    (Catalog Number: GB-116)

    Best For

    Routine protein extraction and purification

    More demanding protein extraction and purification where increased inhibitor strength is desired

    Core Inhibitors

    AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF

    AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF

    Protease Coverage

    Broad-spectrum inhibition of serine, cysteine, and other proteases

    Broad-spectrum inhibition with an enhanced dose of protease inhibitors

    Metalloprotease Inhibition

    Separate EDTA solution included

    Separate EDTA and 1,10-phenanthroline monohydrate solutions included

    EDTA

    Optional

    Optional

    1,10-Phenanthroline

    Not included

    Included separately for additional metalloprotease inhibition

    Metal-Sensitive Applications

    EDTA can be omitted when divalent cations must be preserved

    EDTA and 1,10-phenanthroline can be omitted when divalent cations must be preserved

     

     

     

    Common Applications

    (Click each for more information)

    Protein Extraction and Cell Lysis
    • Purpose: To protect proteins from degradation when cells or tissues are disrupted for protein extraction.
    • How It Works: ProBlock™ Gold combines inhibitors that target multiple protease classes released during lysis. Adding the 100X cocktail to extraction buffer helps limit unwanted proteolysis before proteins can be isolated or analyzed.
    • Applications: Mammalian cell lysis, bacterial and yeast protein extraction, plant and animal tissue homogenization, and preparation of whole-cell lysates.

    Grabski, A. C. (2009). Advances in preparation of biological extracts for protein purification. Methods in Enzymology, 463, 285–303. https://doi.org/10.1016/S0076-6879(09)63018-4

    Recombinant Protein Purification
    • Purpose: To reduce degradation of recombinant proteins during isolation and purification.
    • How It Works: The cocktail inhibits endogenous proteases that can remain active after cell disruption and continue degrading the target protein during subsequent purification steps. The separately supplied EDTA can be omitted when using immobilized metal affinity chromatography.
    • Applications: Affinity chromatography, recombinant protein isolation, enzyme purification, and preparation of proteins for downstream characterization.

    Ryan, B. J., & Henehan, G. T. (2013). Overview of approaches to preventing and avoiding proteolysis during expression and purification of proteins. Current Protocols in Protein Science, 71, 5.25.1–5.25.7. https://doi.org/10.1002/0471140864.ps0525s71

    Proteomics Sample Preparation
    • Purpose: To preserve the protein composition of cell and tissue samples prior to proteomic analysis.
    • How It Works: Broad protease inhibition limits unwanted protein cleavage after sample collection and lysis, helping preserve proteins before subsequent processing and analysis.
    • Applications: Protein extraction for mass spectrometry, quantitative proteomics, protein profiling, and high-throughput proteomic studies, with inhibitor removal or dilution before protease digestion when required.

    Burns, A. P., Zhang, Y. Q., Xu, T., Wei, Z., Yao, Q., Fang, Y., ... & Tao, D. (2021). A universal and high-throughput proteomics sample preparation platform. Analytical Chemistry, 93(24), 8423–8431.

    Preparation of Samples for 2D Electrophoresis and Isoelectric Focusing
    • Purpose: To minimize proteolytic changes to proteins before electrophoretic separation while allowing researchers to avoid EDTA when necessary.
    • How It Works: Protease inhibitors help preserve proteins during sample preparation before electrophoresis. Because EDTA is supplied separately from ProBlock™ Gold, the primary cocktail can be used without the chelator for applications where EDTA could interfere with separation or analysis.
    • Applications: Two-dimensional gel electrophoresis, isoelectric focusing, protein profiling, and comparative proteomic analysis.

    Castellanos-Serra, L., & Paz-Lago, D. (2002). Proteomics and two-dimensional electrophoresis-Inhibition of unwanted proteolysis during sample preparation: Evaluation of its efficiency in challenge experiments. Electrophoresis-An International Journal, 23(11), 1745–1753.

     

    Benefits:

    • Greater Range of Inhibition – 95%+
    • Better than tablets – the ready-to-use, refrigerator-stable, 100X liquid format is more effective and easier to use than traditional tablet products; just pipette exactly the amount you need!
    • No proprietary ingredients – formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A, and PMSF
    • Multiple package sizes
    • EDTA-free formulation is perfect for isoelectric focusing
    • Optional bottle of EDTA is included for optimized metalloprotease inhibition

     

    Storage/Handling: 

    Store at 4°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

    Product Specifications

    Catalog ID: GB-108
    Storage/handling: Store at 4°C.
    UN number: 1993
    group number: III
    item class: None

    CITATIONS

    Featured Articles

      Login

      Forgot your password?

      Don't have an account yet?
      Create account