Description
GoldBio’s Sodium Taurodeoxycholate is a high-purity, water-soluble bile salt detergent widely used in membrane protein research, microbiology, and lipid solubilization studies. As a conjugated bile acid salt, sodium taurodeoxycholate effectively disrupts lipid bilayers and forms mixed micelles, making it ideal for solubilizing and stabilizing membrane proteins, permeabilizing cell membranes, or mimicking bile salt function in lipid transport assays.
It also plays a critical role in selective growth media for enteric bacteria and has proven utility in endotoxin removal workflows.
Common Applications
(Click each for more information)
Solubilization of Membrane Proteins
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Purpose: To extract and stabilize membrane-bound proteins for structural or functional studies.
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How It Works: As an anionic bile salt detergent, sodium taurodeoxycholate disrupts lipid bilayers and forms mixed micelles with membrane proteins, preserving their activity and conformation.
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Applications: Membrane protein purification, receptor-ligand binding assays, ion channel studies.
le Maire, M., Champeil, P., & Møller, J. V. (2000). Interaction of membrane proteins and lipids with solubilizing detergents. Biochim Biophys Acta, 1508(1-2), 86–111. https://doi.org/10.1016/s0304-4157(00)00010-1
Discusses bile salt detergents like taurodeoxycholate and their utility in preserving membrane protein function during solubilization.
Component of Selective Growth Media for Enteric Bacteria
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Purpose: To inhibit the growth of non-enteric organisms while supporting bile salt-tolerant species.
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How It Works: Sodium taurodeoxycholate mimics bile toxicity, enabling the selective culture of organisms like Salmonella and E. coli on bile salt agar or MacConkey-type media.
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Applications: Microbiological diagnostics, enteric pathogen research, selective plating techniques.
MacFaddin, J. F. (1985). Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria (Vol. 1).
Cites taurodeoxycholate in bile-based selective media formulations for isolating gastrointestinal bacteria.
Model Bile Salt in Micelle and Lipid Transport Studies
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Purpose: To investigate bile salt micelle formation, lipid solubilization, and cholesterol transport.
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How It Works: Sodium taurodeoxycholate forms mixed micelles with phospholipids and cholesterol, modeling bile acid-mediated fat absorption.
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Applications: Studies in digestive physiology, micelle behavior, and lipophilic drug solubilization.
Carey, M. C., et al. (1983). Molecular physical chemistry of bile salts. Prog Liver Dis, 8, 239–252.
Describes taurodeoxycholate’s role in bile micelles and its influence on cholesterol and lipid solubilization.
Reagent in Lipopolysaccharide (LPS) and Endotoxin Removal
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Purpose: To disrupt endotoxin–protein complexes and improve endotoxin clearance during protein purification.
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How It Works: Taurodeoxycholate interacts with the hydrophobic regions of LPS aggregates, facilitating dissociation from target proteins.
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Applications: Endotoxin reduction in recombinant protein preps, bioprocessing workflows.
Petsch, D., & Anspach, F. B. (2000). Endotoxin removal from protein solutions. J Biotechnol, 76(2-3), 97–119. https://doi.org/10.1016/S0168-1656(99)00162-4
Discusses bile salts like taurodeoxycholate in disrupting endotoxin–protein associations.
Key Benefits:
Mild yet effective detergent: Solubilizes membrane proteins while preserving native structure and activity.
Versatile in membrane biology: Used for both full solubilization and selective permeabilization.
Ideal for selective microbiological media: Enables targeted growth of bile-resistant enteric organisms.
Mimics physiological bile salts: Useful in lipid digestion, transport, and bile micelle studies.
Enhances endotoxin removal protocols: Improves protein purity in sensitive downstream applications.
Storage/Handling
Store at -20°C in well-sealed container and protect from light.
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