Description
TES is a zwitterionic buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is part of the Tris family of buffers and has a pKa that is right at physiological pH (7.4) at 25°C, giving it a useful pH range of 6.8 – 8.2. It is used in various protein assays, in micellar electrokinetic chromatography and in Test Yolk Buffer medium. Like most neutral pH, amine-based buffers, TES forms a complex with DNA; it affects restriction enzymes kinetics more than HEPES, but less than Tris. It also forms a complex with Cu(II) ions. TES is capable of influencing mRNA expression and embryo development. It is suitable for use in the bicinchoninic acid (BCA) assay.
Product Specifications
TES;
N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid;
ULTRA PURE GRADE
Formula: C6H15NO6S
MW: 229.25 g/mol
PubChem Chemical ID: 81831
Storage/Handling: Store at room temperature.
Video: How to make TES stock solution
Powered by Bioz