TMB dihydrochloride

Name: TMB dihydrochloride
Brand: GoldBio
SKU: TMBHCL10
Price: 816.0 USD

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Description

TMB Dihydrochloride — Chromogenic Substrate for Peroxidase / ELISA Applications

Product Overview

TMB Dihydrochloride (3,3′,5,5′-Tetramethylbenzidine dihydrochloride) is a high-purity, molecular biology grade chromogenic substrate widely used in enzyme-linked immunosorbent assays (ELISAs), immunohistochemistry, blotting, and cytochemical staining. GoldBio’s TMB Dihydrochloride yields a soluble blue oxidation product in the presence of horseradish peroxidase (HRP) and hydrogen peroxide, enabling sensitive colorimetric detection.

Because the reaction can be stopped with acid (turning the color yellow), the assay is compatible with endpoint quantification and is ideal for microplate readers.

Key Specifications & Identity

Parameter	Value / Description
Synonyms / Alternate Names	TMB·HCl, TMB dihydrochloride
CAS Number	207738-08-7
Molecular Formula	C₁₆H₂₀N₂ · 2 HCl (plus hydration)
Approx. Molecular Weight	313.27 g/mol (anhydrous)
Grade	Molecular Biology Grade
Physical Form	Solid (powder)
Storage / Handling	Store desiccated at –20 °C, protected from light

Mechanism of Action & Functional Highlights

In the presence of HRP (horseradish peroxidase) and hydrogen peroxide, TMB dihydrochloride acts as an electron donor / chromogenic substrate. The enzyme catalyzes oxidation, converting TMB into a blue diimine/diamine product.
The blue reaction product has absorbance maxima around 370 nm and 652 nm (in the non-acidified form).
Upon addition of a stop solution (commonly sulfuric acid or a strong acid), the blue oxidized TMB is converted into a yellow species, which is read at ~ 450 nm. This shift permits sensitive endpoint detection in microplate assays.
Because TMB yields a soluble colored product (rather than a precipitate in many standard formulations), it simplifies assay workflows—no solubilization of precipitates is needed.
TMB dihydrochloride is considered a safer alternative to older chromogens like benzidine, being non-carcinogenic / less hazardous.

Suggested Applications & Usage Notes

ELISA / Immunoassays
Use TMB dihydrochloride in HRP-based ELISA protocols to detect antigen, antibody binding, or other analytes. Monitor color development kinetically or stop and read absorbance at 450 nm.
Immunohistochemistry / Blotting / Cytochemistry
Apply TMB in situ for visualization of HRP-labeled probes in tissue sections, membranes, or cells.
Hemoglobin / Peroxidase Quantification
TMB may also be used in colorimetric assays for hemoglobin or endogenous peroxidase enzymes in cells or tissues.

Usage & Handling Tips:

Prepare fresh working solutions and add hydrogen peroxide just before use to avoid background oxidation.
Protect solutions and solid material from light, as TMB is photosensitive and may degrade.
Use matched blank wells (substrate + buffer but no enzyme) to correct for background.
When stopping the reaction, use an acid (e.g. 2 M H₂SO₄) in the correct volume — typical stop volumes ~ 1/10 to 1/4 the reaction volume — then read at 450 nm.
Because TMB dihydrochloride is hydrate / salt form, ensure solubility and buffering conditions (e.g. pH, ionic strength) are compatible with your assay system.
Use proper controls (enzyme negative, substrate only, etc.) to differentiate signal from noise.

Safety & Disclaimer

For research use only. Not for diagnostic or therapeutic use.
Handle with care: wear gloves, eye protection, lab coat, and work in a well-ventilated area.
Avoid inhalation, ingestion, or direct contact with skin or eyes.
Dispose of waste in compliance with institutional and regulatory guidelines.
For complete hazard and handling information, refer to the Safety Data Sheet (SDS).

TMB dihydrochloride

Catalog Number:

TMBHCL10

CAS Number:

207738-08-7

Price:

$816.00

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Description

TMB Dihydrochloride — Chromogenic Substrate for Peroxidase / ELISA Applications

Product Overview

Because the reaction can be stopped with acid (turning the color yellow), the assay is compatible with endpoint quantification and is ideal for microplate readers.

Key Specifications & Identity

Parameter	Value / Description
Synonyms / Alternate Names	TMB·HCl, TMB dihydrochloride
CAS Number	207738-08-7
Molecular Formula	C₁₆H₂₀N₂ · 2 HCl (plus hydration)
Approx. Molecular Weight	313.27 g/mol (anhydrous)
Grade	Molecular Biology Grade
Physical Form	Solid (powder)
Storage / Handling	Store desiccated at –20 °C, protected from light

Mechanism of Action & Functional Highlights

In the presence of HRP (horseradish peroxidase) and hydrogen peroxide, TMB dihydrochloride acts as an electron donor / chromogenic substrate. The enzyme catalyzes oxidation, converting TMB into a blue diimine/diamine product.
The blue reaction product has absorbance maxima around 370 nm and 652 nm (in the non-acidified form).
Upon addition of a stop solution (commonly sulfuric acid or a strong acid), the blue oxidized TMB is converted into a yellow species, which is read at ~ 450 nm. This shift permits sensitive endpoint detection in microplate assays.
Because TMB yields a soluble colored product (rather than a precipitate in many standard formulations), it simplifies assay workflows—no solubilization of precipitates is needed.
TMB dihydrochloride is considered a safer alternative to older chromogens like benzidine, being non-carcinogenic / less hazardous.

Suggested Applications & Usage Notes

ELISA / Immunoassays
Use TMB dihydrochloride in HRP-based ELISA protocols to detect antigen, antibody binding, or other analytes. Monitor color development kinetically or stop and read absorbance at 450 nm.
Immunohistochemistry / Blotting / Cytochemistry
Apply TMB in situ for visualization of HRP-labeled probes in tissue sections, membranes, or cells.
Hemoglobin / Peroxidase Quantification
TMB may also be used in colorimetric assays for hemoglobin or endogenous peroxidase enzymes in cells or tissues.

Usage & Handling Tips:

Prepare fresh working solutions and add hydrogen peroxide just before use to avoid background oxidation.
Protect solutions and solid material from light, as TMB is photosensitive and may degrade.
Use matched blank wells (substrate + buffer but no enzyme) to correct for background.
When stopping the reaction, use an acid (e.g. 2 M H₂SO₄) in the correct volume — typical stop volumes ~ 1/10 to 1/4 the reaction volume — then read at 450 nm.
Because TMB dihydrochloride is hydrate / salt form, ensure solubility and buffering conditions (e.g. pH, ionic strength) are compatible with your assay system.
Use proper controls (enzyme negative, substrate only, etc.) to differentiate signal from noise.

Safety & Disclaimer

For research use only. Not for diagnostic or therapeutic use.
Handle with care: wear gloves, eye protection, lab coat, and work in a well-ventilated area.
Avoid inhalation, ingestion, or direct contact with skin or eyes.
Dispose of waste in compliance with institutional and regulatory guidelines.
For complete hazard and handling information, refer to the Safety Data Sheet (SDS).

Catalog ID:	TMBHCL
CAS #:	207738-08-7
MW:	313.27 g/mol
Grade:	MOLECULAR BIOLOGY GRADE
Storage/handling:	Store desiccated at -20°C. Protect from light.

TMB dihydrochloride

TMB dihydrochloride

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