How to Label Antibodies with Biotin or Fluorophores
Labeling antibodies with biotin or a fluorophore enables scientists to detect, track, and quantify that antibody and the molecules that it binds to. We cover...
Articles
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Why Do Researchers Label Antibodies?
Covalently conjugating a small molecule to an antibody’s surface is a process called antibody “labeling.” Labeling antibodies with small molecules such as biotin or fluorophores...
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Getting Started with DNA Gel Electrophoresis: Your Guide and Introduction
DNA gel electrophoresis is one of the most widely used analytical techniques in molecular biology, providing a simple and reliable way to separate DNA fragments...
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Nickel Affinity Chromatography - The Complete Overview
Do you have a favorite restaurant that you love because you know exactly how great the experience is going to be? There are probably a...
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Best Types of Agarose Gels for DNA Electrophoresis – Buyer’s Guide
Using the wrong agarose can lead to smearing or poor separation. The good news is that once you know how to evaluate agarose and choose...
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How to Make an IPTG Stock Solution
IPTG is frequently used for blue/white colony screening and to induce protein expression in bacterial cultures. Fortunately, IPTG is very stable and easy to work...
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IPTG Induction vs Auto-Induction
I had been optimizing my protein’s expression for months and I thought that I had already tried nearly everything that could be done. Still, I...
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Optimizing Protein Expression with IPTG Induction
Isopropyl b-D-1-thiogalactopyranoside (IPTG) induces recombinant protein expression in bacteria. But have you ever used your lab’s standard IPTG induction protocol and been disappointed with your...
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What Is a His-Tag?
His-tags are popular affinity tags that are used for protein purification, detection, and many different assays. But if you’re new to the protein biochemistry space,...
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How Long Do Competent Cells Last?
You reach into the deep, recesses of your -80 ºC freezer. After some searching, you find it: the competent cells you need. The box of...
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Why Did My Nickel Agarose Beads Lose Their Color?
Nickel agarose beads have a beautiful blue color. This blue color isn’t just for aesthetics; it is also a useful tracker of your purification or...
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Why Did My Nickel Agarose Beads Turn Black or Brown?
If you’ve purified his-tagged proteins with nickel agarose beads, then you know the characteristic brilliant blue color of these beads. When working with those beads,...
