Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
Articles
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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GelRed™ vs GelGreen™: Choosing the Right DNA Gel Stain for Clear Results*
GelRed™ and GelGreen™ are both DNA gel stains designed as safer alternatives to ethidium bromide, with no detectable mutagenicity at concentrations used for DNA gel...
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What are BL21 Competent Cells?
BL21 competent cells are an E. coli strain that is specifically engineered to excel at recombinant protein expression. There are different types of BL21 to...
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10 Easy Tips to Finally Organize Your Laboratory Fridge/Freezer
It’s defrost day in the lab, and you think to yourself, “This might be the best day to organize my fridge and freezers.” Then you...
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The Future of Protein Purification
Breakthroughs in computational predictions are enabling the design of novel proteins for critical medical, environmental, and technical applications. The final “designer proteins” for these applications...
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High-Throughput Methods for Protein Purification
Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
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Breakthroughs in Computational Protein Design
The history of protein and protein purification research stretches back more than 200 years and includes several Nobel prizes (Figure 1). Modern-day scientists use the...
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GelRed vs. Ethidium Bromide: A Comprehensive Look
GelRedTM is a safer, more sensitive alternative to ethidium bromide that uses two ethidium subunits connected by a spacer to prevent cell membrane penetration while...
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Move Aside Dippin’ Dots™: the Next Ice Cream of the Future is Here
There’s nothing quite like cold ice cream on a hot summer day! The race is on to scarf down that delicious treat before it turns...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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What is Forskolin? A Molecular Biologist’s Guide to this Powerful cAMP Modulator
Forskolin comes from the roots of the Coleus forskohlii plant. While it has many broader applications, it is also used across many different disciplines within...
