What Are DH5a Competent Cells?
DH5a competent cells are a genetically engineered Escherichia coli (E. coli) strain designed for high-efficiency DNA transformation. DH5a is a go-to strain for DNA cloning, plasmid propagation,...
Articles
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10 Easy Tips to Finally Organize Your Laboratory Fridge/Freezer
It’s defrost day in the lab, and you think to yourself, “This might be the best day to organize my fridge and freezers.” Then you...
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High-Throughput Methods for Protein Purification
Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
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Breakthroughs in Computational Protein Design
The history of protein and protein purification research stretches back more than 200 years and includes several Nobel prizes (Figure 1). Modern-day scientists use the...
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GelRed vs. Ethidium Bromide: A Comprehensive Look
Your electrophoresis routine has become second nature. You mix the buffer, melt the agarose, and reach for that familiar bottle of ethidium bromide (EtBr). You...
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Move Aside Dippin’ Dots™: the Next Ice Cream of the Future is Here
There’s nothing quite like cold ice cream on a hot summer day! The race is on to scarf down that delicious treat before it turns...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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What is Forskolin? A Molecular Biologist’s Guide to this Powerful cAMP Modulator
Forskolin comes from the roots of the Coleus forskohlii plant. While it has many broader applications, it is also used across many different disciplines within...
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What is a GST Pull-Down Assay? The Complete Overview with Protocols and Analyses Guides
Molecular interactions are foundational for homeostasis at cellular and organism levels, and the dysregulation of these interactions causes many diseases. That’s why when a scientist...
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How to Regenerate Glutathione Resin
Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate...
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How to Prepare Glutathione Elution Buffer
To make glutathione elution buffer, add reduced glutathione powder to your buffer at a final concentration of 10 to 20 millimolar (mM) within an hour...
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What’s the Binding Capacity of Glutathione Agarose
You want to purify Glutathione S-transferase (GST) or a GST-fusion protein. So, you pull your GoldBio glutathione agarose beads out of the refrigerator to start...
