How to Biotinylate Proteins for Experiments and Purification
The protein streptavidin clamps onto the small molecule biotin in one of the tightest natural interactions ever discovered. Over the years, the strength of this...
Articles
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How to Calculate the Amount of Solvent to Add to D-Luciferin
Maybe you are about to start working with D-luciferin, and you begin reviewing protocols. But what you’re doing is a little different. Or what you...
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Everything About Labeling Proteins with NHS Esters
Handles are common in everyday life. We use them to help us open doors, cabinets, or to hold coffee mugs, cookware, and tools. They are...
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How to Label Antibodies with Biotin or Fluorophores
Labeling antibodies with biotin or a fluorophore enables scientists to detect, track, and quantify that antibody and the molecules that it binds to. We cover...
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Why Do Researchers Label Antibodies?
Covalently conjugating a small molecule to an antibody’s surface is a process called antibody “labeling.” Labeling antibodies with small molecules such as biotin or fluorophores...
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Getting Started with DNA Gel Electrophoresis: Your Guide and Introduction
DNA gel electrophoresis is one of the most widely used analytical techniques in molecular biology, providing a simple and reliable way to separate DNA fragments...
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Nickel Affinity Chromatography - The Complete Overview
Do you have a favorite restaurant that you love because you know exactly how great the experience is going to be? There are probably a...
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Best Types of Agarose Gels for DNA Electrophoresis – Buyer’s Guide
Using the wrong agarose can lead to smearing or poor separation. The good news is that once you know how to evaluate agarose and choose...
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How to Make an IPTG Stock Solution
IPTG is frequently used for blue/white colony screening and to induce protein expression in bacterial cultures. Fortunately, IPTG is very stable and easy to work...
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IPTG Induction vs Auto-Induction
I had been optimizing my protein’s expression for months and I thought that I had already tried nearly everything that could be done. Still, I...
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Optimizing Protein Expression with IPTG Induction
Isopropyl b-D-1-thiogalactopyranoside (IPTG) induces recombinant protein expression in bacteria. But have you ever used your lab’s standard IPTG induction protocol and been disappointed with your...
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What Is a His-Tag?
His-tags are popular affinity tags that are used for protein purification, detection, and many different assays. But if you’re new to the protein biochemistry space,...
