Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Articles
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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Featuring the Coolest Floating Tube Rack Creations
The GoldBio Floating Tube Rack is one of our more clever giveaways because of the unique purpose it serves. And, with it also being one...
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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GelRed™ vs GelGreen™: Choosing the Right DNA Gel Stain for Clear Results*
GelRed™ and GelGreen™ are both DNA gel stains designed as safer alternatives to ethidium bromide, with no detectable mutagenicity at concentrations used for DNA gel...
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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The Future of Protein Purification
Breakthroughs in computational predictions are enabling the design of novel proteins for critical medical, environmental, and technical applications. The final “designer proteins” for these applications...
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What are BL21 Competent Cells?
BL21 competent cells are an E. coli strain that is specifically engineered to excel at recombinant protein expression. There are different types of BL21 to...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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What Are DH10B Competent Cells Used For?
DH10B are an engineered strain of Escherichia coli (E. coli) that are highly efficient at molecular cloning. DH10B cells are particularly adept at handling large...
