The Power of Purified Proteins: Medical Applications and Future Possibilities
Think about the last time you were at the doctor’s office or in the hospital. Were you tested for coronavirus or another illness? Did you...
Articles
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The Art and Science of Food Alchemy: Innovations in Enzyme Applications from Cheesemaking to Brewing
Think about the last time you went to a party or a potluck; what did you eat and drink? Was there a cheese platter with...
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4 Experimental Applications of Transfection
Experimental applications using transfection include gene editing, modifying host cell gene expression, and for recombinant protein and viral vector production. Transfection introduces foreign nucleic acids...
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Probing the Depths of Biology: 4 Ways Purified Proteins Drive Research
Our cells are beautifully complex 4D puzzles - consisting of millions of molecular pieces that engage in dynamic interactions to sustain life as we know...
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3 Transfection Methods - A Basic Overview
There are three common transfection methods: chemical, physical and viral. Physical transfection uses physical means such as biolistics, microinjection and lasers. Chemical transfection uses liposomal...
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From Labs to Life: The Versatility of Purified Proteins in Practical Settings
Purified proteins help feed the world, keep us healthy, improve common products, and even begin solving pollution problems. Purified proteins are proteins that have been...
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E. coli Homologous Recombination: How It Works and Influences on Cloning
Homologous recombination is the exchange of sequences that occurs between two similar (homologous) nucleic acid molecules. This happens in both prokaryotes and eukaryotes. The process...
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Understanding Auxotrophic Mutants: Yeast and Bacterial Cloning Tools
Auxotrophic mutants are bacteria, yeast, protoplast or mammalian host cell strains that can’t produce a nutrient vital for growth due to genetic mutations. As a...
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All About the Turbidity-Based Lysozyme Activity Assay
Lysozyme is an enzyme that breaks down peptidoglycan, and it is often used in protein purification, RNA purification and Northern blot to lyse cells in...
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What are E. coli Expression Strains?
E. coli expression strains, BL21(DE3) and DL39 for example, are used for recombinant RNA/protein expression. They have the necessary genetic features such as lacking proteolytic...
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Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10
Alpha-complementation – it’s a big phrase, but it’s not terribly hard to understand. The technical explanation of alpha-complementation involves combining two parts of a protein,...
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