5X Passive Lysis Buffer is a concentrated cell lysis buffer that is designed for use with GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit.The kit is supplied with enough lysis buffer to perform the stated number of assays.This 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer is required.

5X Passive Lysis Buffer is not compatible with Firefly Luciferase or the Illumination™ Luciferase Enhanced Assay Kit.For Firefly luciferase assays, use 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740).

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Plain agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.Plain agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations. Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization. Cross-linked agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

This matrix is a highly crosslinked 6% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure Standard Beads.

This matrix is not preactivated. For preactivated resins, look for Glyoxal Agarose Beads or Protein G.

6-Aminopenicillanic acid (6-APA) is the central component of penicillin β-lactam antibiotics which are generated by Penicillium. 6-APA forms from penicillin G after being catalyzed by penicillin acylases. This compound can be used to create semi-synthetic antibiotics that are effective against non-penicillinase forming bacterium, such as Streptococcus and some Staphylococcus.

6-APA is used as precursor for antibiotic compounds ampicillin and amoxicillin. Antibiotic synthesis of 6-APA can be augmented with a supersaturated solution of nucleophiles. Other methods for enhancing effectiveness involves diminishing ionic strength in a reaction mixture along with the addition of 2-butanol. Implementing a pH gradient starting at pH 7 and reducing the pH to 6.5 near reaction completion can greatly increase reaction output.

6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate is used to detect proteins and amino acids. It reacts with amino acids forming stable unsymmetrical urea derivatives. It can be used with HPLC analysis.

6-azaindole is a reagent used in biochemical synthesis. It can be used as a building block in creating a complex 6-azaindole containing an HIV-1 inhibitor with the prodrug BMS-663068.

6-Azauracil is a growth inhibitor effective on a variety of microorganisms. It functions by depleting the intracellular GTP and UTP nucleotide supply. 6-AU is research of transcription modulation.

The plant hormones cytokinins (CKs) comprise a class of growth regulators involved in the stress response, senescence, photosynthesis, nutrient assimilation and mobilization, as well as modulation of a plant tissue’s ability to act as a sink or source of metabolites.

CKs are recognized by a subset of sensor histidine kinases (HKs) and the signal is transmitted to the nucleus via a multistep phosphorelay (MSP), thus activating the expression of target genes

6-Benzyloxyindole-3-carboxaldehyde is a cyclic reagent for the creation of organic molecules like substituted naphthylene leukotriene B4 antagonists which are useful as anti-inflammatory agents.