GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

AGL-1 (pSoup) chemically competent cells are free of animal-derived products and grown with animal-free media.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications Competent cell type: Chemically Competent Species: A. tumefaciens Strain: AGL-1 (pSoup)Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNABlue/white screening: No

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: AGL-1 Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: EHA105 Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

Product Specifications Competent cell type: Chemically Competent Species: A. tumefaciens Strain: GV3101 Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNABlue/white screening: No

GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: LBA4404 Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s LBA4404 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.

Product Specifications Competent cell type: ElectroCompetent Species: A. tumefaciens Strain: LBA4404 Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA Blue/white screening: No