4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.

GoldBio’s
N-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also known as Amplex Red, is a highly
sensitive fluorogenic substrate used for detecting hydrogen peroxide (H₂O₂) and
monitoring oxidase activity in biochemical and cell-based assays. In the
presence of horseradish peroxidase (HRP), ADHP reacts with H₂O₂ to produce the
intensely fluorescent molecule resorufin, making it ideal for quantifying
glucose, choline, cholesterol, and reactive oxygen species (ROS). With low
background fluorescence, high stability, and excellent sensitivity in the
nanomolar range, ADHP is the gold standard reagent for redox biology, metabolic
profiling, and antioxidant screening. Whether you're conducting enzymatic
assays or oxidative stress research, GoldBio’s ADHP delivers superior
performance, reproducibility, and trusted results in every experiment.

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

Blue Tetrazolium chloride is a chemical compound used for sensitive detection of alkaline phosphatase. With immunohistochemistry blue tetrazolium is used as a diagnostic for diseases of the phagocyte function by gauging the resulting blue color in the presence of oxygen formed by the phagocyte while killing bacteria.

DAB tetrahydrochloride hydrate is a water soluble form of 3-3'-diaminobenzidine and is used to stain for peroxidase activity. The intensity of DAB staining can be increased with the addition of cobalt, nickel or silver ions.

Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.

Horseradish peroxidase (HRP) is the most commonly used antibody-conjugated enzyme. Substrates for HRP are luminol and benzidine substrates. Tetramethylbenzidine (TMB) and similar substrates are oxidized by HRP in the presence of hydrogen peroxide, or nontoxic substitutes, to yield a chinoid structure of intense blue color.

Horseradish peroxidase is a common marker enzyme for antibodies, and can catalyze the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive ELISA assays. Luminescence can be detected at very low levels using extremely sensitive scintillation counters.

Luminol is also used for the detection of blood stains in criminal investigation. For this, luminol is mixed with a liquid containing hydrogen peroxide. If the mixture comes in contact with hemoglobin, the iron in the hemoglobin accelerates chemiluminescent oxidation of luminol.

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Nonfluorescent resazurin, which is reduced by viable cells to red-fluorescent resorufin, can be used to detect the metabolic activity of many cell types from bacteria to higher eukaryotes and is a sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. Resazurin is used in the Resazurin Microtiter Assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis, in cell viability tests, and to assay mitochondrial functions in vitro.

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.