The 3xERRE-luciferase plasmid is designed to study the activity of the Estrogen-Related Receptor (ERR) signaling pathway, specifically its role in transcriptional regulation. This plasmid contains three copies of the Estrogen-Related Receptor Response Element (ERRE), which is a DNA sequence recognized by the estrogen-related receptor (ERR) family of transcription factors. ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer.

The 3xERRE-luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio

The 4xCSL-luciferase plasmid is designed to study the Notch signaling pathway by monitoring the transcriptional activity of CSL (CBF1/Suppressor of Hairless/LAG-1), a key transcription factor in the Notch pathway.

Murine notch homologs (N1-4) undergo presenilin-dependent proteolysis.

The 4xCSL-luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at Goldbio.

The HRE-luciferase plasmid is designed for use in studies related to hypoxia (low oxygen) or oxygen-regulated gene expression. It contains a Hypoxia Response Element (HRE) upstream of a luciferase reporter gene. PTEN regulates p300-dependent hypoxia-inducible factor 1 transcriptional activity through Forkhead transcription factor 3a (FOXO3a).

The HRE-Luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio.

The Lenti-luciferase-P2A-Neo plasmid is a lentiviral vector designed for gene expression studies, particularly in mammalian cells, using luciferase as a reporter gene. The plasmid includes a P2A self-cleaving peptide and Neomycin resistance gene (Neo), which allow for both the expression of luciferase and the selection of successfully transduced cells. The lentiviral backbone ensures stable integration into the host genome, making it useful for long-term studies.

A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.

The pLenti-luciferase-P2A-Neo plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio.

The MSCV IRES Luciferase plasmid is designed to facilitate the study of gene expression and the regulation of specific genes in various biological contexts, particularly in hematopoietic cells (blood cells) and stem cell research. This plasmid incorporates a murine stem cell virus (MSCV) backbone, an internal ribosome entry site (IRES), and a luciferase reporter gene.

The pMSCV IRES Luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio.

The pACYC plasmid series is another set of commonly used cloning vectors in molecular biology and biotechnology. PACYC184 has a p15A origin of replication with chloramphenicol and tetracycline resistance genes. Unlike the pUC or pBR325 vectors, pACYC184 plasmids are characterized by a low copy number. This means fewer copies of the plasmid are present in each bacterial cell, which can be advantageous for cloning and expressing genes that are toxic to the host cells when present in high quantities. pACYC184 has a small size of 4245 bp and is suitable for cloning small to medium-sized DNA fragments. PACYC plasmids are especially useful in experiments where multiple plasmids are required within the same cell, or when cloning genes that are deleterious to host cells at high copy numbers.

The pBR325 plasmid is a classic cloning vector used in molecular biology. It is derived from the pBR322 plasmid, which itself is one of the first and most widely used plasmids for cloning purposes.

The pGL3 BRE Luciferase plasmid is designed to study the activity of the BMP (Bone Morphogenetic Protein) signaling pathway by monitoring transcriptional responses to BMP signaling. The BRE (BMP Response Element) in this plasmid is a specific DNA sequence that is activated by the BMP pathway's SMAD transcription factors.

The pGL3 BRE Luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio.

pUC19 Vector Plasmid is a compact, high-copy cloning vector with Ampicillin resistance and a versatile tool in molecular biology research. pUC19 has 2686 base pairs which harbor a 54 base-pair multiple cloning site (MCS) for efficient cloning with 13 unique restriction enzyme sites, offering flexibility and compatibility with a variety of experimental setups. It is identical to pUC18, except that their MCS are oriented opposite of each other.