PCR Master Mixes

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue.

GoldBio’s Goof-Proof™ Universal Probe Master Mix is a ROX-free, hot-start qPCR master mix optimized for fluorescent probe–based detection, such as SNP genotyping and quantitation. It is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes such as TaqMan®.

This 2X master mix delivers sensitive, high-specificity amplification with minimal background and supports multiplexing across a wide range of instrument platforms that do not require ROX normalization.

With built-in hot-start polymerase, dNTPs, and optimized buffer, Goof-Proof™ simplifies setup and improves reproducibility across both research and diagnostic workflows. Whether you’re running high-throughput assays or targeted qPCR panels, Goof-Proof™ offers the flexibility and precision you need without the interference of passive dyes.

Note: Goof-Proof™ Universal Probe Master Mix does not include ROX reference dye. For instruments that require either a low or high concentration of ROX reference dye, we offer Goof-Proof™ Universal Probe Master Mix with ROX (Catalog # G-715) which includes a separate vial of ROX Reference dye, and instructions for how much ROX to add depending on which protocol and instrument you are using.

GoldBio's Goof-Proof™ Universal Probe Master Mix with ROX is a hot-start 2X qPCR master mix optimized for fluorescent probe-based detection, including SNP genotyping, gene expression analysis, and quantitative PCR.

The included ROX passive reference dye provides signal normalization on compatible real-time PCR instruments, helping compensate for non-PCR-related fluorescence fluctuations and improving data consistency across wells.

Designed for both standard and fast cycling protocols, Goof-Proof™ delivers sensitive, high-specificity amplification with minimal background and excellent performance in both singleplex and multiplex assays. The master mix is compatible with all major fluorescent probe chemistries, including hydrolysis probes such as TaqMan® and displacement probes such as Molecular Beacons.

Note: We also offer Goof-Proof™ Universal Probe Master Mix (Catalog # G-710) without ROX for instruments that do not require the ROX reference dye.