How Biotinylated Molecules are Purified with Streptavidin Beads
Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affinity. This is one of the strongest noncovalent interactions ever...
| Catalog ID: | B-950 | 
|---|---|
| CAS #: | 58-85-5 | 
| MW: | 244.31 g/mol | 
| Grade: | MOLECULAR BIOLOGY GRADE | 
| Storage/handling: | Store at 4°C. | 
        
      Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affinity. This is one of the strongest noncovalent interactions ever...
        
      Eluting biotinylated proteins and DNA from streptavidin beads requires harsh conditions like enzymatic digestion, extreme pH, organic solvents, and heat with excess biotin. Deciding which...
        
      en using streptavidin agarose beads to purify biotinylated nucleic acids or proteins, you’ll need to decide exactly how much resin to use for your purification....