MOPS, Sodium Salt

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Description

MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.

MOPS sodium salt can be mixed with MOPS free acid to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.

Product Specifications
MOPS, Sodium Salt
Sodium;3-(N-morpholino)propanesulfonic acid

HIGH PURITY GRADE

Formula: C7H14NO4SNa

MW: 231.25 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 3859613


Video: How to make a 1M MOPS, Sodium Salt Buffer Stock Solution

MOPS, Sodium Salt

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Catalog Number:
M-791-25
CAS Number:
71119-22-7
$28.00

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    Description

    MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.

    MOPS sodium salt can be mixed with MOPS free acid to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.

    Product Specifications
    MOPS, Sodium Salt
    Sodium;3-(N-morpholino)propanesulfonic acid

    HIGH PURITY GRADE

    Formula: C7H14NO4SNa

    MW: 231.25 g/mol

    Storage/Handling: Store in a dry and well-ventilated place.

    PubChem Chemical ID: 3859613


    Video: How to make a 1M MOPS, Sodium Salt Buffer Stock Solution

    Product Specifications

    Catalog ID: M-791
    CAS #: 71119-22-7
    MW: 231.25 g/mol
    Grade: HIGH PURITY GRADE
    Storage/handling: Store desiccated at room temperature.

    CITATIONS

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