Nickel NTA Agarose Beads

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Description

GoldBio’s Nickel-NTA Agarose Beads are affinity chromatography resins for the purification of polyhistidine-tagged recombinant proteins under native or denaturing conditions.

The resin consists of nickel ions chelated to nitrilotriacetic acid (NTA) groups immobilized on agarose beads, enabling selective binding of histidine-rich protein sequences through metal coordination interactions. Nickel-NTA purification systems are among the most widely used tools in recombinant protein purification because they provide high selectivity, scalability, and compatibility with a broad range of expression systems.

These agarose beads are used in purification procedures involving His-tagged proteins expressed in bacterial, yeast, insect, or mammalian systems. The product supports purification from crude lysates while maintaining compatibility with downstream biochemical, enzymatic, and structural analyses.

Nickel-NTA Agarose Beads are frequently incorporated into protein expression and purification research involving enzyme characterization, structural biology, antibody engineering, and interaction studies.

The agarose support matrix provides good flow characteristics and compatibility with gravity-flow columns, spin columns, and batch purification methods commonly used in molecular biology and protein research.

Nickel-NTA agarose resin is suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.


Note: GoldBio recommends using Nickel NTA resins in the presence of elevated levels of reducing agents in order to ensure optimal purification of your target protein. Nickel IDA resins may show some discoloration in reaction with low levels of reducing agents but are still functional at low levels of reducing agents (≤5mM DTT).

 

Common Applications:

(Click each for more information)

Purification of His-Tagged Recombinant Proteins
  • Purpose: To selectively isolate recombinant proteins containing polyhistidine affinity tags.
  • How It Works: Histidine residues coordinate with nickel ions immobilized on the NTA agarose matrix, enabling selective protein binding.
  • Applications: Recombinant protein purification, enzyme isolation, and protein production studies.

Bornhorst, J. A., & Falke, J. J. (2000). Purification of proteins using polyhistidine affinity tags. Methods in Enzymology, 326, 245–254.

Protein Purification Under Denaturing Conditions
  • Purpose: To recover recombinant proteins from inclusion bodies or insoluble fractions.
  • How It Works: Nickel-NTA beads retain affinity for His-tagged proteins in the presence of denaturants such as urea or guanidine HCl.
  • Applications: Inclusion body purification, protein refolding studies, and insoluble protein recovery.

Block, H., Maertens, B., Spriestersbach, A., et al. (2009). Immobilized-metal affinity chromatography (IMAC): A review. Methods in Enzymology, 463, 439–473.

Structural Biology and Protein Characterization
  • Purpose: To obtain purified proteins suitable for downstream structural and biochemical analyses.
  • How It Works: Selective affinity purification improves protein purity prior to crystallography, NMR, or enzymatic assays.
  • Applications: Protein crystallography, structural biology, and biophysical characterization.

Porath, J. (1992). Immobilized metal ion affinity chromatography. Protein Expression and Purification, 3(4), 263–281.

Protein-Protein Interaction Studies
  • Purpose: To isolate tagged bait proteins and associated binding partners.
  • How It Works: His-tagged proteins are immobilized on Nickel-NTA resin, enabling capture and analysis of interacting proteins.
  • Applications: Pull-down assays, interaction mapping, and binding studies.

Hoffmann, A., & Roeder, R. G. (1991). Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies. Nucleic Acids Research, 19(22), 6337–6338. PMID: 1956786.

High-Throughput Recombinant Protein Screening
  • Purpose: To rapidly purify multiple recombinant proteins in parallel.
  • How It Works: Nickel-NTA agarose supports scalable purification compatible with spin columns, batch formats, and automation.
  • Applications: Protein expression screening, recombinant clone evaluation, and parallel purification methods.

Arnau, J., Lauritzen, C., Petersen, G. E., & Pedersen, J. (2006). Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expression and Purification, 48(1), 1–13.

 

Key Benefits:

  • Highly selective purification of His-tagged recombinant proteins.
  • Compatible with native and denaturing purification conditions.
  • Suitable for batch purification, gravity-flow columns, and spin-column formats.
  • Supports purification from bacterial, yeast, insect, and mammalian expression systems.

 

Storage/Handling:

Store at 4°C. Do not freeze.

 

Nickel NTA Agarose Beads

View Sizes & Pricing

Catalog Number:
H-350-5
CAS Number:
$80.00

For research use only. Not for food, drug, household, or cosmetic use.
Availability:
In stock
Shipping:
$14.99 Ground shipping (In continental US only.)

    Description

    GoldBio’s Nickel-NTA Agarose Beads are affinity chromatography resins for the purification of polyhistidine-tagged recombinant proteins under native or denaturing conditions.

    The resin consists of nickel ions chelated to nitrilotriacetic acid (NTA) groups immobilized on agarose beads, enabling selective binding of histidine-rich protein sequences through metal coordination interactions. Nickel-NTA purification systems are among the most widely used tools in recombinant protein purification because they provide high selectivity, scalability, and compatibility with a broad range of expression systems.

    These agarose beads are used in purification procedures involving His-tagged proteins expressed in bacterial, yeast, insect, or mammalian systems. The product supports purification from crude lysates while maintaining compatibility with downstream biochemical, enzymatic, and structural analyses.

    Nickel-NTA Agarose Beads are frequently incorporated into protein expression and purification research involving enzyme characterization, structural biology, antibody engineering, and interaction studies.

    The agarose support matrix provides good flow characteristics and compatibility with gravity-flow columns, spin columns, and batch purification methods commonly used in molecular biology and protein research.

    Nickel-NTA agarose resin is suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.


    Note: GoldBio recommends using Nickel NTA resins in the presence of elevated levels of reducing agents in order to ensure optimal purification of your target protein. Nickel IDA resins may show some discoloration in reaction with low levels of reducing agents but are still functional at low levels of reducing agents (≤5mM DTT).

     

    Common Applications:

    (Click each for more information)

    Purification of His-Tagged Recombinant Proteins
    • Purpose: To selectively isolate recombinant proteins containing polyhistidine affinity tags.
    • How It Works: Histidine residues coordinate with nickel ions immobilized on the NTA agarose matrix, enabling selective protein binding.
    • Applications: Recombinant protein purification, enzyme isolation, and protein production studies.

    Bornhorst, J. A., & Falke, J. J. (2000). Purification of proteins using polyhistidine affinity tags. Methods in Enzymology, 326, 245–254.

    Protein Purification Under Denaturing Conditions
    • Purpose: To recover recombinant proteins from inclusion bodies or insoluble fractions.
    • How It Works: Nickel-NTA beads retain affinity for His-tagged proteins in the presence of denaturants such as urea or guanidine HCl.
    • Applications: Inclusion body purification, protein refolding studies, and insoluble protein recovery.

    Block, H., Maertens, B., Spriestersbach, A., et al. (2009). Immobilized-metal affinity chromatography (IMAC): A review. Methods in Enzymology, 463, 439–473.

    Structural Biology and Protein Characterization
    • Purpose: To obtain purified proteins suitable for downstream structural and biochemical analyses.
    • How It Works: Selective affinity purification improves protein purity prior to crystallography, NMR, or enzymatic assays.
    • Applications: Protein crystallography, structural biology, and biophysical characterization.

    Porath, J. (1992). Immobilized metal ion affinity chromatography. Protein Expression and Purification, 3(4), 263–281.

    Protein-Protein Interaction Studies
    • Purpose: To isolate tagged bait proteins and associated binding partners.
    • How It Works: His-tagged proteins are immobilized on Nickel-NTA resin, enabling capture and analysis of interacting proteins.
    • Applications: Pull-down assays, interaction mapping, and binding studies.

    Hoffmann, A., & Roeder, R. G. (1991). Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies. Nucleic Acids Research, 19(22), 6337–6338. PMID: 1956786.

    High-Throughput Recombinant Protein Screening
    • Purpose: To rapidly purify multiple recombinant proteins in parallel.
    • How It Works: Nickel-NTA agarose supports scalable purification compatible with spin columns, batch formats, and automation.
    • Applications: Protein expression screening, recombinant clone evaluation, and parallel purification methods.

    Arnau, J., Lauritzen, C., Petersen, G. E., & Pedersen, J. (2006). Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expression and Purification, 48(1), 1–13.

     

    Key Benefits:

    • Highly selective purification of His-tagged recombinant proteins.
    • Compatible with native and denaturing purification conditions.
    • Suitable for batch purification, gravity-flow columns, and spin-column formats.
    • Supports purification from bacterial, yeast, insect, and mammalian expression systems.

     

    Storage/Handling:

    Store at 4°C. Do not freeze.

     

    Product Specifications

    Catalog ID: H-350
    Storage/handling: Store at 4°C. Do NOT freeze.
    UN number: NA-1993
    group number: III

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