High-Throughput Methods for Protein Purification
Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
| Catalog ID: | P-404 | 
|---|---|
| Storage/handling: | Store at 4°C. Do NOT freeze. | 
        
      Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
        
      This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, or Protein L magnetic agarose beads. Protein A, G, and L...
        
      Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
        
      Have you ever seen a cartoon where a huge magnet is used to pull objects into the sky? Usually, the magnet is intended to secretly...
        
      mmunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to isolate a particular protein of interest out of a complex biochemical mixture...
        
      When affinity purifying a protein, one of the first questions you’ll ask yourself is “how much resin do I need for my purification?” As we’ll...
        
      If you’re using Protein A, Protein G, or Protein L agarose beads to purify antibodies, then a key step in this process is eluting the...
        
      One of the key uses for Protein A, Protein G, and Protein L is to purify antibodies for experimental or therapeutic purposes. Agarose beads with...
        
      Affinity purification is a frequently used technique for purifying proteins. During affinity purification, affinity tags or intrinsic properties of the proteins are used to bind...
        
      Protein A is a bacterial protein from the cell wall of Staphylococus aureus that binds to the heavy chains of antibodies. This interaction is leveraged...
        
      Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense when we are infected. Scientists frequently use antibodies as...
        
      Proteins are the verbs of the cellular world. They perform important functions and carry out actions within our cells required for life itself. Over the...