High-Throughput Methods for Protein Purification
Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
| Catalog ID: | P-434 | 
|---|---|
| Storage/handling: | Store at 4°C. Do NOT freeze. | 
        
      Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
        
      This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, or Protein L magnetic agarose beads. Protein A, G, and L...
        
      Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
        
      mmunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to isolate a particular protein of interest out of a complex biochemical mixture...
        
      When affinity purifying a protein, one of the first questions you’ll ask yourself is “how much resin do I need for my purification?” As we’ll...
        
      If you’re using Protein A, Protein G, or Protein L agarose beads to purify antibodies, then a key step in this process is eluting the...
        
      Protein G binds to the heavy chains of antibodies and is used to purify antibodies and support immunoprecipitation (IP) experiments. Protein G interacts with IgG...
        
      Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense when we are infected. Scientists frequently use antibodies as...
        
      Proteins are the verbs of the cellular world. They perform important functions and carry out actions within our cells required for life itself. Over the...