Purifying proteins with traditional methods is a lot of work. To take a protein from cell lysis, through affinity and size exclusion purifications, perhaps with...
The history of protein and protein purification research stretches back more than 200 years and includes several Nobel prizes (Figure 1). Modern-day scientists use the...
To make glutathione elution buffer, add reduced glutathione powder to your buffer at a final concentration of 10 to 20 millimolar (mM) within an hour...