5 Ways to Get Better GelRed Results*
To get the best results when using GelRedTM, perform post-staining, avoid adding GelRedTM in the running buffer, and adjust your dye concentration. Along with these,...
Articles
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What is Glutathione Agarose?
Glutathione is a tripeptide (glutamate – cysteine – glycine) found in many cells that plays an important role in maintaining redox balance and reducing oxidative...
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What is Glutathione?
Glutathione is a tripeptide antioxidant produced by our bodies that plays a really important role in protecting our proteins and DNA from damage caused by...
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How Biotinylated Molecules are Purified with Streptavidin Beads
Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affinity. This is one of the strongest noncovalent interactions ever...
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How to Solubilize Biotin for Streptavidin Elution Buffer
When using streptavidin agarose beads to purify biotinylated nucleic acids or proteins, there are a number of different ways to elute your biotinylated molecules from...
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How to Elute Biotinylated Proteins and Nucleic Acids from Streptavidin Beads
Eluting biotinylated proteins and DNA from streptavidin beads requires harsh conditions like enzymatic digestion, extreme pH, organic solvents, and heat with excess biotin. Deciding which...
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What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*
Expression systems are important tools in molecular biology, enabling researchers to produce proteins for research, diagnostics, and therapeutics. Examples of E. coli protein expression strains...
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How Much Streptavidin Resin Should I use? Binding Capacity has the Answer
en using streptavidin agarose beads to purify biotinylated nucleic acids or proteins, you’ll need to decide exactly how much resin to use for your purification....
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How do I Get my Protein Out of Inclusion Bodies?
Have you ever had a problem where you had to try many different potential solutions before you finally found one that worked? Early in my...
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Understanding Salting In and Salting Out: Salt as a Protein Purification Tool
Salting in is adding salt to a solution to enhance the solubility of a protein, whereas salting out is adding salt to precipitate a protein....
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How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol
This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, or Protein L magnetic agarose beads. Protein A, G, and L...
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Regular vs Magnetic Agarose Beads: Key Differences and Best Uses
Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads for your protein purification or experiment? If so, this...
