4-Methylumbelliferyl-α- D-mannopyranoside is a fluorogenic substrate for α-D-mannosidase, yielding a blue fluorescent solution upon cleavage. It is used for determination of lectin in a very dilute solution by fluorescence quenching, in studies of the kinetics of saccharide binding to the dimer form of concanavalin A or for diagnosis of alpha-mannosidosis by measuring α-mannosidase in plasma.

4-Methylumbelliferyl-β-D-cellobioside acts as a fluorogenic substrate used to measure the activity of (1,4)-β-Glucanase.

(1,4)-β-Glucanase is an enzyme that breaks down glucose by disrupting the glycosidic bonds. This enzyme is also involved in the cell wall synthesis for various fungi.

4-Methylumbelliferyl-β-D-cellobioside can also be used in Cel D Fusion screenings and activity staining in polyacrylamide gels, yielding blue fluorescence as a result.

Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl-β- D-mannopyranoside is a fluorogenic substrate for detection of β-D-mannopyranosidase.

4-Nitroindole is used in the synthesis of a variety of compounds including: indoles, nucleosides, CB2 cannabinoid receptor ligands, CGRP receptor antagonists, anti-angiogenic agents, meridianin derivatives, tryptophan dioxygenase inhibitors, and 1,3,4,5-tetrahydropyrrolo-[4,3,2-de]quinoline.

4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.