4-MU-beta-D-GlcNAc (4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside) is used as a fluorogenic substrate for N-Acetyl-beta-D-glucosaminidase (NAGase), yielding a blue fluorescent solution.

NAGase is an enzyme abundantly found in the renal tubular epithelia. Increased amounts of this enzyme in urine is often associated with various renal diseases. Therefore, interest in measuring its activity using 4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside in order to diagnose renal diseases has grown more popular.

4-Methylumbelliferyl-alpha-L-arabinofuranoside is a substrate used in alpha-L-furanosidase.

4-Methylumbelliferyl-beta-D-galactopyranoside-6-sulfate is a fluorogenic substrate for galactose-6-sulfate sulphatase and diagnosis of Morquio disease.

4-Methylumbelliferyl-beta-D-xylopyranoside is a fluorogenic substrate used to identify beta-D-xylosidase and for Enterobacteriaceae family identification.

4-Methylumbelliferyl-beta-L-fucopyranoside is a substrate for beta-L-fucosidase and yields a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside is a fluorogenic substrate for phosphatases.

4-Methylumbelliferyl-N- acetyl-β-D- galactoside is a fluorogenic substrate for N-acetyl-beta-D-galactosaminidase and can be used for the detection of Candida albicans.

4-Methylumbelliferyl-α- D-galactopyranoside is a fluorogenic substrate for α-galactosidase, yielding a blue fluorescent solution upon cleavage. It is useful in the species differentiation within the family of Enterobacteriaceae and in the localization of α-galactosidase in plants.

4-Methylumbelliferyl-α- L-iduronic acid cyclohexylammonium salt is a fluorogenic soluble enzyme substrate for α-L-iduronidase. α-L-iduronidase is deficient in the genetic disorder Mucopolysaccharidosis type I, also called Hurler syndrome. 4-Methylumbelliferyl-α-L-iduronide is used in the diagnosis of Hurler syndrome as the substrate in an enzymatic assay of α-L-iduronidase activity.

4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate for the identification, characterization and kinetic analysis of α-glucosidase activity. It is routinely used as a substrate for the assay of acid α-glucosidase in the diagnosis of Pompe's disease.