Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Bithionol is an anti-parasitic compound used for oncology research and molecular biology research. It induces apoptosis in cancerous cells occurs through activating proteolytic caspases. These caspases inactivate PARP-1(Poly [ADP-ribose] polymerase 1) which triggers an intrinsic pathway for apoptosis. Intrinsic apoptotic pathways result in compromising mitochondria and in this case by decreasing transmembrane potential. Reactive oxygen species then accumulate and rupture the mitochondria, releasing cytochrome c an apoptotic inducer.

Bithionol has been used to better understand enzyme allosteric regulation and the mechanism of soluble adenylyl cyclase inhibition, a bicarbonate sensor. Bithionol has been shown to occupy the bicarbonate binding site on soluble adenylyl cyclase. It has also been utilized in research to elucidate the role of mitochondrial potassium channels in preventing ischemia reperfusion injuries in mice.

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications Competent cell type: Chemically Competent Derivative of: BL21 (DE3) Species: E. coli Transformation efficiency: ≥5 x 105 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 108 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications Competent cell type: ElectroCompetent Derivative of: BL21 (DE3) Species: E. coli Format: Tubes Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications Competent cell type: Chemically Competent Derivative of: BL21 Species: E. coli Format: Tubes Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA Blue/white screening: Yes

Write with comfort and ease! GoldBio’s black ink pens were carefully selected and tested against other pens for smoothness and comfort while writing. The pen's sleek black and green exterior measures approximately 5.75 inches, providing an ergonomic fit that feels just right in your hand.

Blasticidin S is an aminoacylnucleoside antibiotic isolated from Streptomyces griseochromogenes. It shows high efficacy against both eukaryotic and prokaryotic cells. Aside from its antimicrobial properties, blasticidin has displayed antiviral, antitumor, and antifungal properties and has been used as a selection agent. It has been used to select for cells containing the bls, bsr, or BSD resistance gene as well as HEK293-T cells and HEK-D5 cells. Additionally, blasticidin is effective in preventing cell culture contamination and studying protein synthesis.

Blazin' Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin' Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin' Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ Luminescent Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Blazin' Bright™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Blazin' Bright™ Luminescent UV Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Gels stained with our Blazin' Bright™ Luminescent UV Protein Gel Stain can be visualized rapidly (5-30 minutes) in a single step without fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ Luminescent UV has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Bleomycin A5 hydrochloride is a compound part of the glycopeptide antibiotic family. It is currently used in oncology research for its strong stimulatory and inhibitory effects. Bleomycin A5 greatly reduces telomerase activity, hindering the proliferation of malignant cells. It has also been shown to induce in vivo fibrosis and inflammation by generating peroxynitrites, a strong oxidizing agent.

Bleomycin A5 stimulates downstream apoptosis by increasing cysteine-aspartic acid protease-3 (caspase-3) activity, tumor protein 53 (p53) production and transforming growth factor beta 1 (TGF-beta 1) formation. These proteins induce apoptosis directly or indirectly by halting cell growth.

Bleomycin Sulfate is an anti-cancer agent isolated from Streptomyces verticillus. Bleomycin is a mixture of bleomycin A2 and bleomycin B2, in an approximately 2:1 ratio, respectively. However, the ratio varies by lot number.

Bleomycin is used against Hodgkin’s lymphoma. Its mechanism is not well understood; however, it is though that bleomycin chelates metallic ions causing a decrease in enzyme stability. The enzymes react with oxygen and produce free radicals, ultimately causing single-stranded breaks in deoxyribose sugars of DNA.