4-Methylumbelliferyl cholin phosphate is a fluorogenic substrate.

4-Methylumbelliferyl nonanoate is a fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage. It can be useful for identification of certain Salmonella species.

4-Methylumbelliferyl palmitate is used as a fluorogenic substrate for a subclass of esterases called lipase.

Lipase is an enzyme that assists in the hydrolysis of fats. They have a large role in digestion as well as dietary lipid transport.

Lysosomal acid lipase (LAL), in particular, is required for the hydrolysis of cholesterol esters which are then delivered into the lysosome. Deficiencies in LAL are primarily associated with two diseases: cholesteryl ester storage disease (CESD) and Wolman’s disease (WD). 4-Methylumbelliferyl palmitate has often been used as one of the lipase substrates in studies involving either of these diseases.

Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases.

4-Methylumbelliferyl phosphate (MUP) is a sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatase.

Phosphotase activity has long been studied due to its importance in cellular function. Upon dephosphorylation, MUP yields fluorescent 4-methylumelliferone (4-MU). This reaction has been used to spot PCR amplified products in ELISA and to classify bacteria.

More specifically, MUP can act as an indicator of C. perfringens because of its ability to detect phosphatase which is a highly specific indicator of C. perfringens.

4-MU-beta-D-GlcNAc (4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside) is used as a fluorogenic substrate for N-Acetyl-beta-D-glucosaminidase (NAGase), yielding a blue fluorescent solution.

NAGase is an enzyme abundantly found in the renal tubular epithelia. Increased amounts of this enzyme in urine is often associated with various renal diseases. Therefore, interest in measuring its activity using 4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside in order to diagnose renal diseases has grown more popular.

4-Methylumbelliferyl-alpha-L-arabinofuranoside is a substrate used in alpha-L-furanosidase.

4-Methylumbelliferyl-beta-D-galactopyranoside-6-sulfate is a fluorogenic substrate for galactose-6-sulfate sulphatase and diagnosis of Morquio disease.