4-Methylumbelliferyl-beta-D-xylopyranoside is a fluorogenic substrate used to identify beta-D-xylosidase and for Enterobacteriaceae family identification.

4-Methylumbelliferyl-beta-L-fucopyranoside is a substrate for beta-L-fucosidase and yields a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside is a fluorogenic substrate for phosphatases.

4-Methylumbelliferyl-N- acetyl-β-D- galactoside is a fluorogenic substrate for N-acetyl-beta-D-galactosaminidase and can be used for the detection of Candida albicans.

4-Methylumbelliferyl-α- D-galactopyranoside is a fluorogenic substrate for α-galactosidase, yielding a blue fluorescent solution upon cleavage. It is useful in the species differentiation within the family of Enterobacteriaceae and in the localization of α-galactosidase in plants.

4-Methylumbelliferyl-α- L-iduronic acid cyclohexylammonium salt is a fluorogenic soluble enzyme substrate for α-L-iduronidase. α-L-iduronidase is deficient in the genetic disorder Mucopolysaccharidosis type I, also called Hurler syndrome. 4-Methylumbelliferyl-α-L-iduronide is used in the diagnosis of Hurler syndrome as the substrate in an enzymatic assay of α-L-iduronidase activity.

4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate for the identification, characterization and kinetic analysis of α-glucosidase activity. It is routinely used as a substrate for the assay of acid α-glucosidase in the diagnosis of Pompe's disease.

4-Methylumbelliferyl-α- D-mannopyranoside is a fluorogenic substrate for α-D-mannosidase, yielding a blue fluorescent solution upon cleavage. It is used for determination of lectin in a very dilute solution by fluorescence quenching, in studies of the kinetics of saccharide binding to the dimer form of concanavalin A or for diagnosis of alpha-mannosidosis by measuring α-mannosidase in plasma.

4-Methylumbelliferyl-β-D-cellobioside acts as a fluorogenic substrate used to measure the activity of (1,4)-β-Glucanase.

(1,4)-β-Glucanase is an enzyme that breaks down glucose by disrupting the glycosidic bonds. This enzyme is also involved in the cell wall synthesis for various fungi.

4-Methylumbelliferyl-β-D-cellobioside can also be used in Cel D Fusion screenings and activity staining in polyacrylamide gels, yielding blue fluorescence as a result.