Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl-β- D-mannopyranoside is a fluorogenic substrate for detection of β-D-mannopyranosidase.

6-Fluoro-3-indoxyl-beta-D-galactopyranoside is a biological substrate used as an chromogenic indicator in biochemical assays.

L-Leucine 7-amido-4- methylcoumarin hydrochloride is used in leucine aminopeptidase assays as a fluorgenic substrate, showing greater sensitivity when compared to 4-methoxynapthylamide assays. Aside from the leucine aminopeptidase enzyme, L-Leucine 7-amido-4- methylcoumarin hydrochloride also works as a fluorogenic subtsrate for broad specificity aminopeptidases, such as aminopeptidase M.

The result of enzymatic activity yields a bright blue fluorescent dye: 7-amino-4methylcoumarin (excitation 380nm emission 440nm).

Leucine aminopeptidase enzymes are found across a wide range of organisms including bacteria, plants and animals, and it has been detected in human tissues, with high activity observed in the kidney and liver.

L-Threonine 7-amido-4-methylcoumarin is an AMC fluorgenic substrate.

L-Tyrosine 7-amido-4-methylcoumarin is used as an AMC fluorogenic substrate.

4-Methylumbelliferyl beta-D-glucuronide (MUG) is a fluorogenic substrate for beta-glucuronidase (GUS). Upon cleavage, MUG yields blue fluorescence seen under UV light.

This substrate is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase, which is encoded by uidA. GUS activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.

It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.