Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Plain agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.Plain agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner.Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.Cross-linked agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

This matrix is a highly cross-linked 4% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure standard beads.

This matrix is not preactivated. For preactivated resins, look for Glyoxal Agarose Beads or Protein G.

Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Plain agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.Plain agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations. Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization. Cross-linked agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.

This matrix is a highly crosslinked 6% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure Standard Beads.

This matrix is not preactivated. For preactivated resins, look for Glyoxal Agarose Beads or Protein G.

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

GoldBio’s Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.

Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.

Ficin is a cysteine protease enzyme which is derived from the latex of fig trees with activity similar to other cysteine protease enzymes including endopeptidases, aminopeptidases, and dipeptidyl peptidases. Ficin cleaves mouse immunoglobulin G molecules (IgG1) in the hinge region. The concentration of cysteine changes how ficin cleaves IgG1. A F(ab’)2 fragment is formed in the presence of 1mM cysteine. The F(ab’)2 fragments are smaller than whole IgG molecules and better penetrate tissue for antigen recognition while avoiding unspecific binding to Fc receptors. In the presence of 10mM cysteine, two 2 Fab fragments are produced instead. F(ab) fragments block background from primary antibody binding, and block endogenous and exposed immunoglobulins and tissues in labeling experiments.

Immobilized Ficin Agarose Resin offers a convenient solution to the production of F(ab’)2 and Fab fragments while eliminating autolysis and protease contamination and the need to remove the enzymes following digestion. Fab and F(ab’)2 can be further separated from whole IgG with either Protein A Agarose Resin (GoldBio Product # P-400) or ion exchange chromatography.

Activity:1-1.5 mg ficin/ml of resin

Support: 6% Cross-linked Agarose

Storage/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Papain is a cysteine protease enzyme from papaya latex that has a wide variety of activities including endopeptidases, aminopeptidases, dipeptidyl peptidases and enzymes with both exo- and endo-peptidase activity. In particular, papain cleaves immunoglobulin G (IgG) molecules near the hinge region of the antibody, resulting in three similar, ~50 kDa, fragments; an Fc domain and two monovalent Fab domains. This papain-digested antibody is therefore unable to promote agglutination, precipitation, opsonization or lysis. The Fc fragment can then be used to determine the specificity of the Fc receptors without any typical antigen binging interference while the Fab fragments can be used in immunohistochemical studies.

Pepsin, one of the principal digestion enzymes, breaks down proteins in to smaller peptides. It is most efficient in cleaving the peptide bonds between hydrophobic and aromatic amino acids such as phenylalanine, tryptophan and tyrosine. Pepsin readily cleaves immunoglobulin G (IgG) molecules, generating F(ab’)2 fragments and small Fc peptide fragments. The smaller Fc fragments can be easily removed via gel filtration while the F(ab’)2 fragments can then be purified from any remaining, undigested IgG. The F(ab’)2 fragments are highly useful for research interests, being able to penetrate tissue better than standard IgG antibodies and facilitate better antigen recognition while avoiding unspecific binding to Fc receptors.

Immobilized Pepsin Agarose Resin is an efficient method for producing F(ab’)2 fragments from IgG molecules for antibody mimetic. Undigested IgG can be quickly removed with our Protein A Agarose Resin (GoldBio Catalog # P-400). Fc fragments can also be removed using a gel filtration system.

Resin: 6% Cross-linked Agarose

Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.