GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

AGL-1 (pSoup) chemically competent cells are free of animal-derived products and grown with animal-free media.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications Competent cell type: Chemically Competent Species: A. tumefaciens Strain: AGL-1 (pSoup)Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNABlue/white screening: No

GoldBio’s AGL-1 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications Competent cell type: Chemically Competent Species: A. tumefaciens Strain: AGL-1 (pSoup-P19)Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNABlue/white screening: No

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: AGL-1 Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications Competent cell type: Chemically Competent Derivative of: BL21 (DE3) Species: E. coli Transformation efficiency: ≥5 x 105 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications Competent cell type: Chemically Competent Derivative of: BL21 Species: E. coli Format: Tubes Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio CJ236 chemically competent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut- and ung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.

CJ236 chemically competent E. coli cells are free of animal-derived products and grown with animal-free media.

Kit Components

• Competent Cells
• 1 x 12 mL Recovery Media
• 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: EHA105 Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s EHA105 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications Competent cell type: Electrocompetent Species: A. tumefaciens Strain: EHA105 Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are equivalent to DH5-alpha cells and are high efficiency cells suitable for transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

GB5-alpha™ chemically competent cells are free of animal-derived products and grown with animal-free media.

GB5-alpha™ Chemically competentE. colicells are equivalent to DH5-alpha.

GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

Product Specifications Competent cell type: Chemically Competent Species: A. tumefaciens Strain: GV3101 Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNABlue/white screening: No