The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

Gibberellic Acid 4 + 7 is a growth regulator that acts by stimulating rapid stem and root growth, induces mitotic division in the leaves of some plants and increase seed germination rate.

The Hygromycin B EZ Pak™ is the fastest and easiest way to make a set amount of sterile hygromycin B solution. The kit includes pre-weighed hygromycin B powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio hygromycin B and the sterile solution is ready for tissue culture, bacterial media or any other appropriate use.

GoldBio’s Illumination™ Firefly Luciferase Enhanced Assay Kit is specifically designed for the simple and efficient quantification of firefly luciferase reporter enzyme activity from cultured cells with the highest degree of sensitivity, reliability and linearity. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.Our Enhanced Assay Kit includes our improved 5X Luciferase Lysis Buffer which provides a better working solution stability compared to other firefly assay kits.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Luciferase Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740) can be purchased separately.

Kit components

• 5X Luciferase Lysis Buffer
• Firefly Luciferase Assay Buffer
• GoldBio D-Luciferin

GoldBio's 6x Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

4-Methylumbelliferyl beta-D-glucuronide (MUG) is a fluorogenic substrate for beta-glucuronidase (GUS). Upon cleavage, MUG yields blue fluorescence seen under UV light.

This substrate is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase, which is encoded by uidA. GUS activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.

It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.

Spectinomycin is an aminocyclitol antibiotic isolated from Streptomyces spectabilis. It is effective against gram-positive and gram-negative bacteria, but most commonly used against Gonococcus species. It has also been used to monitor cell fate during leaf development and as a selection marker in plants to display successful transformation of the Spcr gene.

Spectinomycin functions by binding the 30s ribosomal subunit, inhibiting tRNA translation and protein synthesis. Resistance to this antibiotic is conferred through the aadA gene and mutated rpsE gene.

GoldBio’s antibiotic solutions are provided ready to use.

Simple Stop™ 2 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Mitomycin C is an antineoplastic antibiotic isolated from Streptomyces caespitosus. It is effective against gram-negative and gram-positive bacteria and a variety of cancer cells, including Ehrlich ascites tumor cells. Mitomycin C has been used to produce mitotically inactivated feeder cells and has been shown to increase recombination during fruit fly meiosis. It is capable of killing mutant strains of Arabidopsis that are defective for recombination during meiosis.

Mitomycin C functions as a DNA crosslinking agent, inhibiting DNA synthesis.

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.