GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.

Product Specifications Competent cell type: Chemically competent Derivative of: HB101 Species: E. coli Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA Blue/white screening: No

Heparin-binding epidermal growth factor-like growth factor (HBEGF) is synthesized as a transmembrane precursor that is cleaved by a protease to release HBEGF as the ectodomain. Its expression stimulates neurogenesis and is increased after the brain experiences cerebral hypoxia. HBEGF is known to participate in a variety of normal physiological processes such as blastocyst implantation and wound healing, as well as in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HBEGF is a glycoprotein exhibiting mitogenic and chemotactic activity in smooth muscle cells and fibroblasts.

Heparin-binding epidermal growth factor-like growth factor (HBEGF) is synthesized as a transmembrane precursor that is cleaved by a protease to release HBEGF as the ectodomain. Its expression stimulates neurogenesis and is increased after the brain experiences cerebral hypoxia. HBEGF is known to participate in a variety of normal physiological processes such as blastocyst implantation and wound healing, as well as in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HBEGF is a glycoprotein exhibiting mitogenic and chemotactic activity in smooth muscle cells and fibroblasts.

Heparin-binding epidermal growth factor-like growth factor (HBEGF) is synthesized as a transmembrane precursor that is cleaved by a protease to release HBEGF as the ectodomain. Its expression stimulates neurogenesis and is increased after the brain experiences cerebral hypoxia. HBEGF is known to participate in a variety of normal physiological processes such as blastocyst implantation and wound healing, as well as in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HBEGF is a glycoprotein exhibiting mitogenic and chemotactic activity in smooth muscle cells and fibroblasts.

Hemin is an iron source with an iron-containing porphyrin ring. It is used to study conjugation and toxicity of lipophosphoglycan-polyacrylic acid conjugate for its potential as a vaccine against leishmaniasis.

Hepatitis B virus (HBV) is an infectious, and potentially fatal, human disease that causes serious liver disease. There are three start codons on the Gene S HBV surface protein antigen (HBsAg) that divide the gene into three sections: pre-S1, pre-S2, and S. These start sites create three carboxyl, coterminal polypeptides that are called large (LHB, the order from surface to the inside: pre-S1, pre-S2, and S), middle (MHB, containing the pre-S2 and S portion) and small (SHB, covering only the S portion). The pre-S1 amino acid region of LHBs consists of 119 amino acids and has been shown to interact with its human binding partner, γ2-adaptin, to subvert the membrane-trafficking apparatus to mediate virion export. HBsAg-PreS1 can be used for Immunochromatography, monoclonal and polyclonal antibody preparation or for ELISA analysis.

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

Hexadecylpyridinium bromide is a cationic surfactant capable of bactericidal activity and has been used for ion exchange. As a bactericidal agent, it reduces the surface tension between pathogenic membranes and their environments. This interaction disrupts membrane integrity and osmoregulation, leading to cell lysis.

Hexadecylpyridinium bromide has also been shown to quickly and efficiently remove metal ions from solutions. The compound removes cations from solution through a cation/surfactant complex which precipitates from an aqueous phase into an organic phase and can subsequently be extracted. Hexadecylpyridinium bromide is an effective ion exchange agent for extracting DNA and proteins.