GoldBio’s Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our EHA105 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s EHA105 Agrobacterium electrocompetent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.

Product Specifications Competent cell type: ElectroCompetent Species: A. tumefaciens Strain: EHA105∆Met Format: Tubes Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s new Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: LBA4404∆Met Format: Tubes Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

GoldBio’s Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when dual selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

Product Specifications Competent cell type: ElectroCompetent Species: A. tumefaciens Strain: LBA4404∆Met Format: Tubes Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

Azelaic acid is a dicarboxylic acid found in cereals and animal products. It is bactericidal against Proprionibacterium acnes and Staphylococcus epidermidis by inhibiting synthesis in microbial cellular proteins. It is found to be useful in controlling several skin conditions, including acne and hyperpigmentation. In melanoma cells azelaic acid inhibits DNA synthesis, leading to a significant reduction in proliferation.

Azithromycin is a broad-spectrum macrolide antibiotic similar in structure to erythromycin. It is effective against various gram-negative and gram-positive bacteria. It has also displayed efficacy against certain Mycoplasma species. Azithromycin also exhibits anti-inflammatory and immunosuppressive properties. Azithromycin dihydrate is slightly soluble in aqueous solution.

Macrolide antibiotics are composed of a macrocyclic lactone ring attached to one or more deoxy sugars. They inhibit bacterial protein synthesis by irreversibly binding the P site of the 50s ribosome, preventing peptide bond formation and translocation.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

Azlocillin is an extended-spectrum β-lactam antibiotic derived from ampicillin. It is useful against P. aeruginosa, E. coli, H. influenzae, and a variety of other gram-negative species. It has also been used to develop antibiotic counting techniques. Azlocillin sodium is freely soluble in aqueous solution.

Aztreonam is a monobactam β-lactam antibiotic whose structure contains only a single ring. Its unique ring structure makes it resistant to β-lactamases. Aztreonam is effective against gram-negative bacteria and is an economical antibiotic commonly used in plant tissue culture applications and against P. aeruginosa.

Aztreonam solubilized is blend of aztreonam and 42-45% L-Arginine, making the product freely soluble in water. Aztreonam is a monobactam β-lactam antibiotic whose structure contains only a single ring. Its unique ring structure makes it resistant to β-lactamases. Aztreonam is effective against gram-negative bacteria and is an economical antibiotic commonly used in plant tissue culture applications and against P. aeruginosa.

Bacitrac99 is the most pure form of bacitracin available, and should be used for the most sensitive applications and on the most sensitive cell lines since any impurities that might cause inhibition of resistant cell lines have been removed. In general, our USP Grade bacitracin will not adversely affect your cells and is suitable for most experiments.

Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties.

Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.

Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties. Bacitracin is freely soluble in aqueous solution.

Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.

Bactenecin is a twelve residue antimicrobial peptide that is recognized by its disulfide linked cyclical structure. It lyses gram-negative bacteria by increasing permeability through binding lipopolysaccharides (LPS). The bactenecin/LPS conjugate forms a β-hairpin structures which integrate with the bacterial membrane, forming pores. Cytosol contents leak with pore formation and lysis occurs with higher bactenecin concentrations

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

GoldBio's original formula for Bacterial Cell Lysis Buffer has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. This lysis buffer eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.