Kanamycin Monosulfate 50 mg/mL Solution
Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
GoldBio’s antibiotic solutions are provided ready to use.
Kanamycin Monosulfate in H2O
(50 mg/mL Premade Solution, 0.2 µm Sterile Filtered)
MOLECULAR BIOLOGY GRADE
Formula: C18H36N4O11 · H2SO4
MW: 582.58 g/mol
Storage/Handling: Store at -20°C
Activity: ~800 µg/mg
PubChem Chemical ID: 32943
|Grade||MOLECULAR BIOLOGY GRADE|
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Potato-Agrobacterium Transformation using Kanamycin, Timentin and Zeatin - by Hui Duan et al. (2012)
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines