Competent Cells and Transformation

GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.

  • GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
  • Our enhanced recovery medium (SOC) formulation accompanies all of GoldBio's competent cells.
  • We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
  • Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
  • GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
  • Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
  • GoldBio's amber suppressor (SupE) and non-amber suppressor (MC1061F\’) strains are ideal for phage display library screening.

CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!

Our DH5-alpha, BL21, BL21(DE3), DH10B, DH10B-Pro, DL39(DE3), Agrobacterium, L. lactis, SS320 and TG1 Phage Display cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!

Transformation Efficiency Calculator
Enter your transformation data to calculate transformation efficiency
Calculate
Use GoldBio's Competent Cell Guide to Select the Right Competent Cells for your Assay
Includes transformation efficiencies, genotype, strain information, screening methods and applicatio...
Read more!

GoldBio Competent Cells Consistently Exhibit Higher Transformation Efficiencies

DH10B chemically competent E. coli cells and DH10B Electrocompetent E. coli cells exhibit higher transformation efficiency than competitors. Each transformation with DH10B chemically competent E. coli cells was performed using 1 ml of final volume including recovery media, plating 50 μl after a 10 fold-dilution (left). For electrocompetent cell comparison, DH10B Electrocompetent E. coli cells were electroporated using a 1 ml of final volume including recovery media, plating 50 μl after 100-fold dilution (right).


BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES

Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.

Electroporation and Electrocompetent Cells FAQs:

What is electroporation?

Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.

When should I use electrocompetent cells?

Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.

How do I prevent arcing during electroporation?

Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.

Showing 1-20 of 20 items.

AGL-1 Agrobacterium Electrocompetent Cells

Catalog ID Size Pricing
CC-208-A 6 x 50 µl $ 300.00
CC-208-B 18 x 50 µl $ 650.00

Description

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells are optimized to produce the highest transformation efficiency, and are ideal for applications requiring high transformation efficiencies such as cDNA or gDNA library construction. The AGL-1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL-1 contains the Ti plasmid pTiBo542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.

Note: GoldBio agrobacterial strains have not been comprehensively studied for antibiotic resistance. We recommend using kanamycin for selection against agrobacterial strains, but it remains the researcher’s responsibility to make sure that vectors are compatible with the agrobacterial strains for alternative antibiotic selection.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Quick Add
CC-208-A 6 x 50 µl $ 300.00
CC-208-B 18 x 50 µl $ 650.00

Agrobacterium ElectroCompetent Cell Combo Pack

Catalog ID Size Pricing
CC-230-A 12 x 50 µl $ 815.00

Description

GoldBio’s Agrobacterium ElectroCompetent Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research.The Agrobacterium ElectroCompetent Combo Pack contains three, 50 µl aliquots, of each: GV3101, AGL1, EHA105 and LBA4404 strains, along with pCambia and our proprietary Agro Recovery Media.

View
Quick Add
CC-230-A 12 x 50 µl $ 815.00

BL21 (DE3) Chemically Competent E. coli Cells

There is no image for BL21 (DE3) Chemically Competent E. coli Cells BL21 (DE3) Chemically Competent E. coli Cells
Catalog ID Size Pricing
CC-103-B 12 x 50 μl $ 120.00
CC-103-C 6 x 200 μl $ 145.00
CC-103-D 24 x 50 μl $ 200.00
CC-103-E 12 x 200 μl $ 270.00

Description

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are suitable for transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-103-B 12 x 50 μl $ 120.00
CC-103-C 6 x 200 μl $ 145.00
CC-103-D 24 x 50 μl $ 200.00
CC-103-E 12 x 200 μl $ 270.00

BL21 (DE3) Electrocompetent E. coli Cells

There is no image for BL21 (DE3) Electrocompetent E. coli Cells BL21 (DE3) Electrocompetent E. coli Cells
Catalog ID Size Pricing
CC-204-A 12 x 50 μl $ 225.00
CC-204-B 12 x 100 μl $ 400.00

Description

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature >1 x 1010 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-204-A 12 x 50 μl $ 225.00
CC-204-B 12 x 100 μl $ 400.00

BL21 Chemically Competent E. coli Cells

There is no image for BL21 Chemically Competent E. coli Cells BL21 Chemically Competent E. coli Cells
Catalog ID Size Pricing
CC-102-B 24 x 50 μl $ 190.00

Description

GoldBio’s BL21 Chemically Competent E. coli cells are suitable for transformation and routine protein expression from non-T7 vectors. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-102-B 24 x 50 μl $ 190.00

Competent Cell Recovery Medium

There is no image for Competent Cell Recovery Medium Competent Cell Recovery Medium
Catalog ID Size Pricing
CC-300-A 6 x 12 ml $ 80.00

Description

GoldBio’s Competent Cell Recovery Medium has a superior formulation recommended for all competent cell transformation. Our Recovery Medium is formulated to achieve the highest possible transformation efficiency from any competent cell.

  • Achieve the highest possible transformation efficiency from any competent cell
  • Enhanced SOC formulation provided with all of Intact Genomics’ competent cells
View
Quick Add
CC-300-A 6 x 12 ml $ 80.00

DH10B Chemically Competent E. coli Cells

There is no image for DH10B Chemically Competent E. coli Cells DH10B Chemically Competent E. coli Cells
Catalog ID Size Pricing
CC-100-B 6 x 100 μl $ 115.00
CC-100-C 12 x 50 μl $ 125.00
CC-100-D 6 x 200 μl $ 165.00
CC-100-E 12 x 100 μl $ 195.00
CC-100-F 24 x 50 μl $ 205.00
CC-100-G 12 x 200 μl $ 310.00
CC-100-H 24 x 100 μl $ 330.00
CC-100-J 1 plate (96 x 20 μl) $ 465.00

Description

GoldBio’s DH10B Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. DH10B Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: Chemically competent
Derivative of: DH10B™
Species: E. coli
Format: Tubes or Plate (96 x 20 µl)
Transformation efficiency: ≥1 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-100-B 6 x 100 μl $ 115.00
CC-100-C 12 x 50 μl $ 125.00
CC-100-D 6 x 200 μl $ 165.00
CC-100-E 12 x 100 μl $ 195.00
CC-100-F 24 x 50 μl $ 205.00
CC-100-G 12 x 200 μl $ 310.00
CC-100-H 24 x 100 μl $ 330.00
CC-100-J 1 plate (96 x 20 μl) $ 465.00

DH10B Electrocompetent E. coli Cells

There is no image for DH10B Electrocompetent E. coli Cells DH10B Electrocompetent E. coli Cells
Catalog ID Size Pricing
CC-200-A 6 x 50 μl $ 125.00
CC-200-B 6 x 100 μl $ 195.00
CC-200-C 12 x 50 μl $ 240.00
CC-200-D 12 x 100 μl $ 350.00

Description

GoldBio’s DH10B Electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 1010 cfu/µg plasmid DNA, which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. DH10B Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Format: Tubes
Transformation efficiency: ≥5 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

Product Specifications

Catalog ID CC-200
Storage/Handling Store Competent Cells at -80°C.
View
Quick Add
CC-200-A 6 x 50 μl $ 125.00
CC-200-B 6 x 100 μl $ 195.00
CC-200-C 12 x 50 μl $ 240.00
CC-200-D 12 x 100 μl $ 350.00

DH10B-Pro™ Electrocompetent E. coli Cells

There is no image for DH10B-Pro™ Electrocompetent E. coli Cells DH10B-Pro™ Electrocompetent E. coli Cells
Catalog ID Size Pricing
CC-201-A 6 x 100 µl $ 325.00

Description

GoldBio’s DH10B-Pro™ Electrocompetent E. coli cells are suitable for demanding cloning applications requiring the greatest number of transformants, including synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets. Utilizing proprietary manufacturing methods, these cells allow for effective transformation of all large DNA molecules (≥10 kb up to 350 kb!).

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Format: Tubes
Transformation efficiency: ≥5 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-201-A 6 x 100 µl $ 325.00

DH5-alpha Chemically Competent E. coli Cells

There is no image for DH5-alpha Chemically Competent E. coli Cells DH5-alpha Chemically Competent E. coli Cells
Catalog ID Size Pricing
CC-101-B 6 x 100 μl $ 105.00
CC-101-C 12 x 50 μl $ 120.00
CC-101-D 6 x 200 μl $ 145.00
CC-101-E 12 x 100 μl $ 185.00
CC-101-F 24 x 50 μl $ 195.00
CC-101-G 12 x 200 μl $ 270.00
CC-101-H 24 x 100 μl $ 315.00
CC-101-J 1 plate (96 x 20 μl) $ 465.00

Description

GoldBio’s DH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DH5-alpha
Species: E. coli
Format: Tubes or Plates (96 x 20 µl)
Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-101-B 6 x 100 μl $ 105.00
CC-101-C 12 x 50 μl $ 120.00
CC-101-D 6 x 200 μl $ 145.00
CC-101-E 12 x 100 μl $ 185.00
CC-101-F 24 x 50 μl $ 195.00
CC-101-G 12 x 200 μl $ 270.00
CC-101-H 24 x 100 μl $ 315.00
CC-101-J 1 plate (96 x 20 μl) $ 465.00

DH5-alpha Electrocompetent E. coli Cells

There is no image for DH5-alpha Electrocompetent E. coli Cells DH5-alpha Electrocompetent E. coli Cells
Catalog ID Size Pricing
CC-203-A 6 x 50 μl $ 115.00
CC-203-B 6 x 100 μl $ 175.00
CC-203-C 12 x 50 μl $ 210.00
CC-203-D 12 x 100 μl $ 335.00

Description

GoldBio’s DH5-alpha Electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH5-alpha
Species: E. coli
Format: Tubes
Transformation efficiency: ≥2 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-203-A 6 x 50 μl $ 115.00
CC-203-B 6 x 100 μl $ 175.00
CC-203-C 12 x 50 μl $ 210.00
CC-203-D 12 x 100 μl $ 335.00

DL39 (DE3) Chemically Competent E. coli Cells

There is no image for DL39 (DE3) Chemically Competent E. coli Cells DL39 (DE3) Chemically Competent E. coli Cells
Catalog ID Size Pricing
CC-104-B 6 x 200 μl $ 465.00

Description

GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label residues (e.g. phenylalanine or leucine). DL39 (DE3) Chemically Competent E. coli cells can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylananine, tyrosine and tryptophan residues.These cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases. These cells also contain the T7 expression system.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-104-B 6 x 200 μl $ 465.00

EHA105 Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-225-A 6 x 50 µl $ 300.00
CC-225-B 18 x 50 µl $ 650.00

Description

GoldBio’s EHA105 ElectroComp Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

Note: GoldBio agrobacterial strains have not been comprehensively studied for antibiotic resistance. We recommend using kanamycin for selection against agrobacterial strains, but it remains the researcher’s responsibility to make sure that vectors are compatible with the agrobacterial strains for alternative antibiotic selection.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Quick Add
CC-225-A 6 x 50 µl $ 300.00
CC-225-B 18 x 50 µl $ 650.00

GV3101 Agrobacterium Electrocompetent Cells

Catalog ID Size Pricing
CC-207-A 6 x 50 µl $ 300.00
CC-207-B 18 x 50 µl $ 650.00

Description

GoldBio’s GV3101 Agrobacterium Electrocompetent Cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as cDNA or gDNA library construction. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) with gentamicin resistance. GV3101 is also resistant to Chloramphenicol and this antibiotic cannot be used for selection. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant S genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato and soybeans as well as monocots like corn.

Note: GoldBio agrobacterial strains have not been comprehensively studied for antibiotic resistance. We recommend using kanamycin for selection against agrobacterial strains, but it remains the researcher’s responsibility to make sure that vectors are compatible with the agrobacterial strains for alternative antibiotic selection.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Quick Add
CC-207-A 6 x 50 µl $ 300.00
CC-207-B 18 x 50 µl $ 650.00

i7® High-Fidelity DNA Polymerase 2X Master Mix

There is no image for i7® High-Fidelity DNA Polymerase 2X Master Mix i7® High-Fidelity DNA Polymerase 2X Master Mix
Catalog ID Size Pricing
D-922-100 100 Reactions $ 175.00
D-922-500 500 Reactions $ 599.00

Description

i7® High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
  • Efficient for amplifying high GC content template DNA with GC enhancer.
View
Quick Add
D-922-100 100 Reactions $ 175.00
D-922-500 500 Reactions $ 599.00

i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix

There is no image for i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix
Catalog ID Size Pricing
D-923-100 100 Reactions $ 195.00
D-923-500 500 Reactions $ 609.00

Description

i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains hot start i7® high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
  • Efficient for amplifying high GC content template DNA with GC enhancer.
View
Quick Add
D-923-100 100 Reactions $ 195.00
D-923-500 500 Reactions $ 609.00

Lactococcus lactis IL1403 Electrocompetent Cells

There is no image for Lactococcus lactis IL1403 Electrocompetent Cells Lactococcus lactis IL1403 Electrocompetent Cells
Catalog ID Size Pricing
CC-210-A 12 x 50 μl $ 395.00

Description

Lactococcus lactis IL1403 Electrocompetent Cells are customer strains optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

Product Specifications
Competent cell type: ElectroCompetent
Species: Lactococcus lactis
Strain: IL1403
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pNZ8148 DNA
Blue/white screening: No

View
Quick Add
CC-210-A 12 x 50 μl $ 395.00

Lactococcus lactis MG1363 Electrocompetent Cells

There is no image for Lactococcus lactis MG1363 Electrocompetent Cells Lactococcus lactis MG1363 Electrocompetent Cells
Catalog ID Size Pricing
CC-209-A 12 x 50 μl $ 395.00

Description

Lactococcus lactis MG1363 Electrocompetent Cells are customer strains optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

Product Specifications
Competent cell type: ElectroCompetent
Species: Lactococcus lactis
Strain: MG1363
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pNZ8148 DNA
Blue/white screening: No

View
Quick Add
CC-209-A 12 x 50 μl $ 395.00

LBA4404 Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-220-A 6 x 50 µl $ 280.00
CC-220-B 18 x 50 µl $ 650.00

Description

GoldBio’s LBA4404 ElectroComp Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.LBA4404 contains a rifampicin resistance gene (rif) and also has streptomycin resistance.LBA4404 strain also contains an octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.

Note: GoldBio agrobacterial strains have not been comprehensively studied for antibiotic resistance. We recommend using kanamycin for selection against agrobacterial strains, but it remains the researcher’s responsibility to make sure that vectors are compatible with the agrobacterial strains for alternative antibiotic selection.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Quick Add
CC-220-A 6 x 50 µl $ 280.00
CC-220-B 18 x 50 µl $ 650.00

TG1 Phage Display Electrocompetent Cells

There is no image for TG1 Phage Display Electrocompetent Cells TG1 Phage Display Electrocompetent Cells
Catalog ID Size Pricing
CC-205-A 6 x 100 μl $ 185.00
CC-205-B 12 x 100 μl $ 325.00

Description

GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥4 x 1010 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).

Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥4 x 1010 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Quick Add
CC-205-A 6 x 100 μl $ 185.00
CC-205-B 12 x 100 μl $ 325.00

Join our list to receive promos and articles.

Join Now