Polymerase chain reaction (PCR), clustered regularly interspaced short palindromic repeats (CRISPR) and DNA gel electrophoresis are powerful molecular biology techniques. Here at GoldBio, we understand the reagents and enzymes you need to perform these techniques successfully in your lab must be of the highest quality. Therefore, we have developed a collection of superior products including dNTPs, DNA polymerases, reverse transcriptases, ligases, master mixes, PCR and quantitative PCR kits, protocols and tools to help you achieve your research goals.
PCR Products & Resources
Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.
Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.
CRISPR Products & Resources
The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.
DNA Gel Electrophoresis Products & Resources
Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).
Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.