DNA Amplification

Polymerase chain reaction (PCR), clustered regularly interspaced short palindromic repeats (CRISPR) and DNA gel electrophoresis are powerful molecular biology techniques. Here at GoldBio, we understand the reagents and enzymes you need to perform these techniques successfully in your lab must be of the highest quality. Therefore, we have developed a collection of superior products including dNTPs, DNA polymerases, reverse transcriptases, ligases, master mixes, PCR and quantitative PCR kits, protocols and tools to help you achieve your research goals.

Featuring: Probe-Based qPCR Kits
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PCR Products & Resources

Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.

Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.

PCR Overview
PCR involves the use of DNA polymerase to synthesize complementary strands of DNA from a template...
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CRISPR Products & Resources

The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.



DNA Gel Electrophoresis Products & Resources

Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).

Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.


Showing 1-9 of 9 items.

Bsu DNA Polymerase Large Fragment

There is no image for Bsu DNA Polymerase Large Fragment Bsu DNA Polymerase Large Fragment
Catalog ID Size Pricing
B-095-1000 1000 units $ 259.00

Description

Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.

Applications

  • Strand displacement DNA synthesis
  • Random primer labeling
  • Second strand cDNA synthesis
  • dA-tailing
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B-095-1000 1000 units $ 259.00

Hot Start Taq 2x Master Mix - 20 µL reaction

There is no image for Hot Start Taq 2x Master Mix - 20 µL reaction Hot Start Taq 2x Master Mix - 20 µL reaction
Catalog ID Size Pricing
T-513-100 100 reactions $ 45.00
T-513-500 500 reactions $ 145.00

Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Specifications

Catalog ID T-513
Storage/Handling Store at -20°C.
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T-513-100 100 reactions $ 45.00
T-513-500 500 reactions $ 145.00

Hot Start Taq 2x Master Mix - 50 µL reaction

There is no image for Hot Start Taq 2x Master Mix - 50 µL reaction Hot Start Taq 2x Master Mix - 50 µL reaction
Catalog ID Size Pricing
T-512-100 100 reactions $ 78.00
T-512-500 500 reactions $ 216.00

Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

  • Hot Start Taq 2x master mix
  • 5x GC Enhancer

Taq Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Taq Concentration (1x): 0.1 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Product Specifications

Catalog ID T-512
Storage/Handling Store at -20°C.
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T-512-100 100 reactions $ 78.00
T-512-500 500 reactions $ 216.00

i7® High-Fidelity DNA Polymerase 2X Master Mix

There is no image for i7® High-Fidelity DNA Polymerase 2X Master Mix i7® High-Fidelity DNA Polymerase 2X Master Mix
Catalog ID Size Pricing
D-922-100 100 Reactions $ 175.00
D-922-500 500 Reactions $ 599.00

Description

i7® High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
  • Efficient for amplifying high GC content template DNA with GC enhancer.
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D-922-100 100 Reactions $ 175.00
D-922-500 500 Reactions $ 599.00

i7® Hot Start High-Fidelity DNA Polymerase

There is no image for i7® Hot Start High-Fidelity DNA Polymerase i7® Hot Start High-Fidelity DNA Polymerase
Catalog ID Size Pricing
D-921-200 200 Units $ 199.00
D-921-500 500 Units $ 399.00

Description

i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. i7 Hot Start High-Fidelity DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. i7® Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 2.5X PCR reaction buffer containing MgCl2 with a final (1X) concentration of 2mM. This buffer allows for amplification of non GC rich templates as well as GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
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D-921-200 200 Units $ 199.00
D-921-500 500 Units $ 399.00

i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix

There is no image for i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix
Catalog ID Size Pricing
D-923-100 100 Reactions $ 195.00
D-923-500 500 Reactions $ 609.00

Description

i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains hot start i7® high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
  • Efficient for amplifying high GC content template DNA with GC enhancer.
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D-923-100 100 Reactions $ 195.00
D-923-500 500 Reactions $ 609.00

InstantAmp™ Viral Solution for Covid-19

There is no image for InstantAmp™ Viral Solution for Covid-19 InstantAmp™ Viral Solution for Covid-19
Catalog ID Size Pricing
I-305-100 100 reactions $ 349.00
I-305-2000 2,000 reactions $ 6,195.00

Description

GoldBio’s InstantAmp™ Viral Solution for Covid-19 can be utilized for saliva/swab samples resulting in viral/cell lysis, and significantly simplifying this process. This solution streamlines viral specimen collection and viral RNA preparation processes including sample collection, transport, maintenance, viral/cell lysis, and viral RNA stabilization. After using the solution, the lysed viral RNA may be directly used in RT-qPCR, RT-isothermal DNA amplification, or any direct diagnosis kits for Covid-19 testing, without the need of an RNA extraction step.

InstantAmp™ Viral/Cell Solution for Covid-19 Testing can be used to detect SARS-CoV-2 in any type of respiratory specimens such as swabs, saliva, sputum, nasopharyngeal, oropharyngeal aspirates, washes or/and tracheal aspirates.

This solution allows for efficient viral sample collection, processing, and SARS-CoV-2 viral RNA preparation in a single tube.

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I-305-100 100 reactions $ 349.00
I-305-2000 2,000 reactions $ 6,195.00

PCR Cloning Kit

There is no image for PCR Cloning Kit PCR Cloning Kit
Catalog ID Size Pricing
P-910-10 10 Reactions $ 229.00
P-910-50 50 Reactions $ 799.00
P-910-100 100 Reactions $ 1,299.00

Description

Our PCR Cloning kit utilizes cloning technology that is simple, rapid and highly efficient which allows direct cloning of any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by our high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 minutes) with more than 95% cloning efficiency.

Applications

  • Clone any insert at any site within any vector
  • Restriction enzyme and phosphatase free system
  • Joining multiple large fragments at once
  • Precise insertion at a desired orientation
  • Rapid and high efficiency with >95% positive clones
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P-910-10 10 Reactions $ 229.00
P-910-50 50 Reactions $ 799.00
P-910-100 100 Reactions $ 1,299.00

Sau DNA Polymerase Large Fragment

There is no image for Sau DNA Polymerase Large Fragment Sau DNA Polymerase Large Fragment
Catalog ID Size Pricing
S-095-1000 1000 units $ 499.00

Description

Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.

Applications

  • Strand displacement DNA synthesis
  • Random primer labeling
  • Second strand cDNA synthesis
  • dA-tailing
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S-095-1000 1000 units $ 499.00

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